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Anti-Tenascin Polyclonal Antibody is a Rabbit antibody targeting Tenascin. Anti-Tenascin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-Tenascin Polyclonal Antibody is a Rabbit antibody targeting Tenascin. Anti-Tenascin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
Alias | TNC, TN, Tenascin-C (TN-C), Tenascin, Neuronectin, Myotendinous antigen, JI, HXB, Hexabrachion, GP 150-225, GMEM, Glioma-associated-extracellular matrix antigen, Cytotactin |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Pig) |
Verified Activity | 1. Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Tenascin C/Tn-C Polyclonal Antibody, Unconjugated (TMAB-01814) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Tenascin C/Tn-C Polyclonal Antibody, Unconjugated (TMAB-01814) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
Application | |
Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
Construction | Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle. |
Immunogen | KLH conjugated synthetic peptide: human Tn-C |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | ECM Proteins,Neural Signal Transduction,Secreted Molecules,Extracellular |
Molecular Weight | Theoretical: 239 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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