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Anti-PYCARD Polyclonal Antibody

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Catalog No. TMAB-01595

Anti-PYCARD Polyclonal Antibody is a Rabbit antibody targeting PYCARD. Anti-PYCARD Polyclonal Antibody can be used in WB.

Anti-PYCARD Polyclonal Antibody

Anti-PYCARD Polyclonal Antibody

😃Good
Catalog No. TMAB-01595
Anti-PYCARD Polyclonal Antibody is a Rabbit antibody targeting PYCARD. Anti-PYCARD Polyclonal Antibody can be used in WB.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$372 7-10 days
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Instruction Manual of Detection Antibody
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Product Introduction

Bioactivity
Description
Anti-PYCARD Polyclonal Antibody is a Rabbit antibody targeting PYCARD. Anti-PYCARD Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human
Verified Activity
Sample:
Lane 1: Spleen (Rat) Lysate at 40 μg
Lane 2: U937 (Human) Cell Lysate at 30 μg
Lane 3: THP-1 (Human) Cell Lysate at 30 μg
Primary:
Anti-ASC (TMAB-01595) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 24 kDa
Application
WB
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Note=Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundPromotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Tissue specificity: Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma.
Antigen Details
Immunogen
Recombinant Protein: human ASC
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Tumor biomarkers,Associated Proteins,CARD Family
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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