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Anti-PRKG1 Polyclonal Antibody
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Catalog No. TMAB-01572
Alias PRKGR1B, PRKGR1A, PRKG1B, PRKG1, cGMP-Dependent Protein Kinase I, cGMP-Dependent Protein Kinase 1, cGKI, cGK1, cGK 1
Anti-PRKG1 Polyclonal Antibody is a Rabbit antibody targeting PRKG1. Anti-PRKG1 Polyclonal Antibody can be used in WB,ICC/IF.
Anti-PRKG1 Polyclonal Antibody
Anti-PRKG1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01572Alias PRKGR1B, PRKGR1A, PRKG1B, PRKG1, cGMP-Dependent Protein Kinase I, cGMP-Dependent Protein Kinase 1, cGKI, cGK1, cGK 1
Anti-PRKG1 Polyclonal Antibody is a Rabbit antibody targeting PRKG1. Anti-PRKG1 Polyclonal Antibody can be used in WB,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-PRKG1 Polyclonal Antibody is a Rabbit antibody targeting PRKG1. Anti-PRKG1 Polyclonal Antibody can be used in WB,ICC/IF. |
| Alias | PRKGR1B, PRKGR1A, PRKG1B, PRKG1, cGMP-Dependent Protein Kinase I, cGMP-Dependent Protein Kinase 1, cGKI, cGK1, cGK 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Sample: Hela Cell Lysate at 40 μg U2OS Cell Lysate at 40 μg Primary: Anti-cGKI (TMAB-01572) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 74 kDa 2. Sample: Heart (Mouse) Lysate at 40 μg Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-cGKI (TMAB-01572) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kDa Observed band size: 74 kDa 3. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (cGKI) polyclonal Antibody, Unconjugated (TMAB-01572) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Colocalized with TRPC7 in the plasma membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. PKG plays an important stimulatory role in platelet activation. |
| Immunogen | KLH conjugated synthetic peptide: human Prkg1/cGKI |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Platelets,Hypertrophy,Other Kinases |
| Molecular Weight | Theoretical: 74 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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