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Anti-PRKG1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01572
Alias PRKGR1B, PRKGR1A, PRKG1B, PRKG1, cGMP-Dependent Protein Kinase I, cGMP-Dependent Protein Kinase 1, cGKI, cGK1, cGK 1

Anti-PRKG1 Polyclonal Antibody is a Rabbit antibody targeting PRKG1. Anti-PRKG1 Polyclonal Antibody can be used in WB,ICC/IF.

Anti-PRKG1 Polyclonal Antibody

Anti-PRKG1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01572Alias PRKGR1B, PRKGR1A, PRKG1B, PRKG1, cGMP-Dependent Protein Kinase I, cGMP-Dependent Protein Kinase 1, cGKI, cGK1, cGK 1
Anti-PRKG1 Polyclonal Antibody is a Rabbit antibody targeting PRKG1. Anti-PRKG1 Polyclonal Antibody can be used in WB,ICC/IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-PRKG1 Polyclonal Antibody is a Rabbit antibody targeting PRKG1. Anti-PRKG1 Polyclonal Antibody can be used in WB,ICC/IF.
AliasPRKGR1B, PRKGR1A, PRKG1B, PRKG1, cGMP-Dependent Protein Kinase I, cGMP-Dependent Protein Kinase 1, cGKI, cGK1, cGK 1
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Sample:
Hela Cell Lysate at 40 μg
U2OS Cell Lysate at 40 μg
Primary: Anti-cGKI (TMAB-01572) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
2. Sample:
Heart (Mouse) Lysate at 40 μg
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-cGKI (TMAB-01572) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
3. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (cGKI) polyclonal Antibody, Unconjugated (TMAB-01572) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
Application
WB,ICC/IF
Recommended Dose
WB: 1:500-2000; ICC/IF: 1:100
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Colocalized with TRPC7 in the plasma membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundProtein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. PKG plays an important stimulatory role in platelet activation.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Prkg1/cGKI
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Platelets,Hypertrophy,Other Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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