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Anti-POD1 Polyclonal Antibody
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Catalog No. TMAB-01555
Alias Transcription factor 21, TCF-21, TCF21, Podocyte-expressed 1, POD1, POD 1, Epicardin, Class A basic helix-loop-helix protein 23, Capsulin, bHLHa23
Anti-POD1 Polyclonal Antibody is a Rabbit antibody targeting POD1. Anti-POD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-POD1 Polyclonal Antibody
Anti-POD1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01555Alias Transcription factor 21, TCF-21, TCF21, Podocyte-expressed 1, POD1, POD 1, Epicardin, Class A basic helix-loop-helix protein 23, Capsulin, bHLHa23
Anti-POD1 Polyclonal Antibody is a Rabbit antibody targeting POD1. Anti-POD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-POD1 Polyclonal Antibody is a Rabbit antibody targeting POD1. Anti-POD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Transcription factor 21, TCF-21, TCF21, Podocyte-expressed 1, POD1, POD 1, Epicardin, Class A basic helix-loop-helix protein 23, Capsulin, bHLHa23 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-POD1 Polyclonal Antibody, Unconjugated (TMAB-01555) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-POD1 Polyclonal Antibody, Unconjugated (TMAB-01555) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: K562 (Human) Cell Lysate at 30 μg Primary: Anti-POD1 (TMAB-01555) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 23 kDa 4. Sample: Cerebrum (Mouse) Lysate at 40 μg Kidney (Rat) Lysate at 40 μg Primary: Anti-POD1 (TMAB-01555) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 23 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The basic helix-loop-helix (bHLH) class of transcription factors govern cell fate determination by controlling a variety of cellular differentiation processes. POD-1 (podocyte-expressed 1, also designated capsulin or epicardin) is a nuclear bHLH protein that is involved in the specification of select mesodermal cell populations associated with heart, cranial skeletal muscle, gut and urogenital system. POD-1 is selectively expressed in mesenchymal cells at sites of epithelial-mesenchymal interaction in the kidney, lung, intestine, pancreas and the epicardium, which gives rise to the coronary arteries. This epithelial-mesenchymal interaction is involved in the formation of numerous internal organs. POD-1 is also expressed in the mesothelium that gives rise to the spleen and in cells that give rise to smooth muscle. In addition to its role in kidney morphogenesis and spleen organogenesis, POD-1 may play a role in the development and sex determination of the mammalian gonad. |
| Immunogen | KLH conjugated synthetic peptide: human TCF-21 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Transcription factors/regulators,HLH,Transcription Factors |
| Molecular Weight | Theoretical: 20 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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