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Anti-POD1 Polyclonal Antibody

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Catalog No. TMAB-01555
Alias Transcription factor 21, TCF-21, TCF21, Podocyte-expressed 1, POD1, POD 1, Epicardin, Class A basic helix-loop-helix protein 23, Capsulin, bHLHa23

Anti-POD1 Polyclonal Antibody is a Rabbit antibody targeting POD1. Anti-POD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-POD1 Polyclonal Antibody

Anti-POD1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01555Alias Transcription factor 21, TCF-21, TCF21, Podocyte-expressed 1, POD1, POD 1, Epicardin, Class A basic helix-loop-helix protein 23, Capsulin, bHLHa23
Anti-POD1 Polyclonal Antibody is a Rabbit antibody targeting POD1. Anti-POD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3727-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-POD1 Polyclonal Antibody is a Rabbit antibody targeting POD1. Anti-POD1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasTranscription factor 21, TCF-21, TCF21, Podocyte-expressed 1, POD1, POD 1, Epicardin, Class A basic helix-loop-helix protein 23, Capsulin, bHLHa23
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-POD1 Polyclonal Antibody, Unconjugated (TMAB-01555) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-POD1 Polyclonal Antibody, Unconjugated (TMAB-01555) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
K562 (Human) Cell Lysate at 30 μg
Primary: Anti-POD1 (TMAB-01555) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 23 kDa
4. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Kidney (Rat) Lysate at 40 μg
Primary: Anti-POD1 (TMAB-01555) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 23 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe basic helix-loop-helix (bHLH) class of transcription factors govern cell fate determination by controlling a variety of cellular differentiation processes. POD-1 (podocyte-expressed 1, also designated capsulin or epicardin) is a nuclear bHLH protein that is involved in the specification of select mesodermal cell populations associated with heart, cranial skeletal muscle, gut and urogenital system. POD-1 is selectively expressed in mesenchymal cells at sites of epithelial-mesenchymal interaction in the kidney, lung, intestine, pancreas and the epicardium, which gives rise to the coronary arteries. This epithelial-mesenchymal interaction is involved in the formation of numerous internal organs. POD-1 is also expressed in the mesothelium that gives rise to the spleen and in cells that give rise to smooth muscle. In addition to its role in kidney morphogenesis and spleen organogenesis, POD-1 may play a role in the development and sex determination of the mammalian gonad.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human TCF-21
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Transcription factors/regulators,HLH,Transcription Factors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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