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Anti-PKA alpha/beta Polyclonal Antibody
😃Good
Catalog No. TMAB-01543
Anti-PKA alpha/beta Polyclonal Antibody is a Rabbit antibody targeting PKA alpha/beta. Anti-PKA alpha/beta Polyclonal Antibody can be used in WB,ICC/IF.
Anti-PKA alpha/beta Polyclonal Antibody
Anti-PKA alpha/beta Polyclonal Antibody
😃Good
Catalog No. TMAB-01543
Anti-PKA alpha/beta Polyclonal Antibody is a Rabbit antibody targeting PKA alpha/beta. Anti-PKA alpha/beta Polyclonal Antibody can be used in WB,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-PKA alpha/beta Polyclonal Antibody is a Rabbit antibody targeting PKA alpha/beta. Anti-PKA alpha/beta Polyclonal Antibody can be used in WB,ICC/IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Jurkat (Human) Cell Lysate at 40 μg Primary: Anti-PKA alpha + beta (TMAB-01543) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa 2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PKA alpha + beta) polyclonal Antibody, Unconjugated (TMAB-01543) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 3. Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Rat Liver tissue lysates Primary: Anti-PKA alpha + beta (TMAB-01543) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa |
| Application | WB,ICC/IF |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | oplasm. Cell membrane. Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity). |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PKA beta (catalytic subunit) is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. |
| Immunogen | KLH conjugated synthetic peptide: human PRKACA/PRKACB |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Integration of energy metabolism,Metabolism of lipids and lipoproteins,Serine/threonine kinases,Integration of energy,Lipid metabolism,Cancer,Kinases,PKA,PKC |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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