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Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody

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Catalog No. TMAB-01521

Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TBK1 (Ser172). Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody

Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody

😃Good
Catalog No. TMAB-01521
Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TBK1 (Ser172). Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$373 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TBK1 (Ser172). Anti-Phospho-TBK1 (Ser172) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Rat,Mouse,Human
Verified Activity
1. Sample:
Lane 1: Human Raji cell lysates
Lane 2: Human HeLa cell lysates
Primary: Anti-Phospho-TBK1 (Ser172) (TMAB-01521) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (TMAB-01521) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (TMAB-01521) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (TMAB-01521) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (TMAB-01521) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-TBK1 (Ser172) Polyclonal Antibody, Unconjugated (TMAB-01521) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human TBK1 around the phosphorylation site of Ser172
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
NFkB pathway,Other Kinases,NFkB Pathway
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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