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Anti-Phospho-PRKAR2A (Ser99) Antibody (9R534)

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Catalog No. TMAC-03247
Alias PRKAR2A (p-Ser99), PRKAR2A (p-S99), p-PRKAR2A (Ser99), p-PRKAR2A (S99)

Anti-Phospho-PRKAR2A (S99) Antibody (9R534) is a Rabbit antibody targeting Phospho-PRKAR2A (S99). Anti-Phospho-PRKAR2A (S99) Antibody (9R534) can be used in WB,ICC/IF,IHC,IP.

Anti-Phospho-PRKAR2A (Ser99) Antibody (9R534)

Anti-Phospho-PRKAR2A (Ser99) Antibody (9R534)

😃Good
Catalog No. TMAC-03247Alias PRKAR2A (p-Ser99), PRKAR2A (p-S99), p-PRKAR2A (Ser99), p-PRKAR2A (S99)
Anti-Phospho-PRKAR2A (S99) Antibody (9R534) is a Rabbit antibody targeting Phospho-PRKAR2A (S99). Anti-Phospho-PRKAR2A (S99) Antibody (9R534) can be used in WB,ICC/IF,IHC,IP.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-PRKAR2A (Ser99) Antibody (9R534) is a Rabbit antibody targeting Phospho-PRKAR2A (Ser99). Anti-Phospho-PRKAR2A (Ser99) Antibody (9R534) can be used in WB,ICC/IF,IHC,IP.
SynonymsPRKAR2A (p-Ser99), PRKAR2A (p-S99), p-PRKAR2A (Ser99), p-PRKAR2A (S99)
Ig Type
IgG
Clone
9R534
Reactivity
Human,Mouse,Rat,Pig
Verified Activity
1. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.
4. ICC staining phospho-PKA R2 (S99) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining phospho-PKA R2 (S99) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Recommended Dose
WB: 1:500; IHC: 1:50-200; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionHybridoma Monoclonal Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundThe second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Phospho
Antigen Details
Immunogen
A synthesized phosphopeptide: human PKA R2 around the phosphorylation site of Ser99
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 51 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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