Anti-Phospho-JAK1 (Tyr1034, 1035) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JAK1 (Tyr1034, 1035). Anti-Phospho-JAK1 (Tyr1034, 1035) Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Pig,Cow)

1. Sample: A549 (Human) cell Lysate at 40 μg
Primary: Anti-p-Jak1 (Tyr1034 + Tyr1035)(TMAB-01439) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 133 kDa
2. From 《Cancer Medicine》(2016.6): PublitionDirect effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes , IF:2.5
Author: Noriyoshi Iriyama, Yoshihiro Hatta & Masami Takei
Division of Hematology and Rheumatology, Department of Medicine, Nihon University School of Medicine, Tokyo, Japan
3. Blank control: HUVEC.
Primary Antibody (green line): Rabbit Anti-Phospho-Jak1 antibody (TMAB-01439)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 2μg /Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Jak1 around the phosphorylation site of Tyr1034 + Tyr1035

Human

Signal transducers,Tyrosine kinases,Kinases,STATs,Tyrosine Kinases