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Anti-Phospho-IRF7 (Ser471, 472) Polyclonal Antibody
😃Good
Catalog No. TMAB-01437
Alias p-IRF7 (Ser471, 472), p-IRF7 (S471, 472), IRF7 (p-Ser471, 472), IRF7 (p-S471, 472), IRF-7, IRF7, Interferon regulatory factor 7
Anti-Phospho-IRF7 (Ser471, 472) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IRF7 (Ser471, 472). Anti-Phospho-IRF7 (Ser471, 472) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-Phospho-IRF7 (Ser471, 472) Polyclonal Antibody
Anti-Phospho-IRF7 (Ser471, 472) Polyclonal Antibody
😃Good
Catalog No. TMAB-01437Alias p-IRF7 (Ser471, 472), p-IRF7 (S471, 472), IRF7 (p-Ser471, 472), IRF7 (p-S471, 472), IRF-7, IRF7, Interferon regulatory factor 7
Anti-Phospho-IRF7 (Ser471, 472) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IRF7 (Ser471, 472). Anti-Phospho-IRF7 (Ser471, 472) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-IRF7 (Ser471, 472) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IRF7 (Ser471, 472). Anti-Phospho-IRF7 (Ser471, 472) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Alias | p-IRF7 (Ser471, 472), p-IRF7 (S471, 472), IRF7 (p-Ser471, 472), IRF7 (p-S471, 472), IRF-7, IRF7, Interferon regulatory factor 7 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig,Cow,Horse) |
| Verified Activity | 1. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) polyclonal Antibody, Unconjugated (TMAB-01437) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (TMAB-01437) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (TMAB-01437) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Sample: Lane 1: Mouse Spleen tissue lysates Lane 2: Mouse Thymus tissue lysates Lane 3: Mouse Blood cell lysates Lane 4: Mouse Lung tissue lysates Lane 5: Rat Spleen tissue lysates Lane 6: Rat Liver tissue lysates Lane 7: Human Jurkat cell lysates Lane 8: Human Raji cell lysates Primary: Anti-Phospho-IRF7 (Ser471 + Ser472) (TMAB-01437) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 70,55 kDa |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Note=The phosphorylated and active form accumulates selectively in the nucleus. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthesised phosphopeptide: human IRF7 around the phosphorylation site of Ser471/472 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Helix-Turn-Helix,Pol II Transcription,Antiviral Signaling,Interferons,TLR Signaling,Host Virus Interaction,SARS Coronavirus |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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