Anti-Phospho-IRF3 (Ser386) Antibody (1U230) is a Rabbit antibody targeting Phospho-IRF3 (Ser386). Anti-Phospho-IRF3 (Ser386) Antibody (1U230) can be used in ICC/IF, WB.

IgG

1U230

Human

MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-IRF3 (S386)) monoclonal Antibody, Unconjugated (TMAB-01435) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

ICC/IF=1:100-500; WB=1:500-1000

Monoclonal

KLH conjugated synthesised phosphopeptide: human IRF3 around the phosphorylation site of Ser386

Human

IRF3

Interferon regulatory factor 3

SARS Coronavirus,TLR Signaling,Interferons,Other factors

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.