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Anti-Phospho-IRF3 (Ser386) Antibody (1U230)

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Catalog No. TMAB-01435
Alias p-IRF3 (Ser386), p-IRF3 (S386), Phospho-IRF3 (S386), MGC94729, IRF3 (p-Ser386), IRF3 (p-S386), IRF 3, Interferon regulatory factor 3, IIAE7

Anti-Phospho-IRF3 (Ser386) Antibody (1U230) is a Rabbit antibody targeting Phospho-IRF3 (Ser386). Anti-Phospho-IRF3 (Ser386) Antibody (1U230) can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.

Anti-Phospho-IRF3 (Ser386) Antibody (1U230)

Anti-Phospho-IRF3 (Ser386) Antibody (1U230)

😃Good
Catalog No. TMAB-01435Alias p-IRF3 (Ser386), p-IRF3 (S386), Phospho-IRF3 (S386), MGC94729, IRF3 (p-Ser386), IRF3 (p-S386), IRF 3, Interferon regulatory factor 3, IIAE7
Anti-Phospho-IRF3 (Ser386) Antibody (1U230) is a Rabbit antibody targeting Phospho-IRF3 (Ser386). Anti-Phospho-IRF3 (Ser386) Antibody (1U230) can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.
Pack SizePriceAvailabilityQuantity
25 μL$1537-10 days
50 μL$2737-10 days
100 μL$4887-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-IRF3 (Ser386) Antibody (1U230) is a Rabbit antibody targeting Phospho-IRF3 (Ser386). Anti-Phospho-IRF3 (Ser386) Antibody (1U230) can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.
Synonymsp-IRF3 (Ser386), p-IRF3 (S386), Phospho-IRF3 (S386), MGC94729, IRF3 (p-Ser386), IRF3 (p-S386), IRF 3, Interferon regulatory factor 3, IIAE7
Ig Type
IgG
Clone
1U230
Reactivity
Human (predicted:Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep)
Verified Activity
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-IRF3 (S386)) monoclonal Antibody, Unconjugated (TMAB-01435) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
Application
Recommended Dose
WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:50-200; ICC/IF: 1:50; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
Tissue SpecificityExpressed constitutively in a variety of tissues.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundThis gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011].
Antigen Details
Immunogen
KLH conjugated synthesised phosphopeptide: human IRF3 around the phosphorylation site of Ser386
Antigen Species
Human
Gene Name
IRF3
Gene ID
Protein Name
Interferon regulatory factor 3
Uniprot ID
Biology Area
SARS Coronavirus,TLR Signaling,Interferons,Other factors
Function
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Chemical Properties
Molecular WeightTheoretical: 47 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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