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Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody
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Catalog No. TMAB-01404
Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody is a Rabbit antibody targeting Phospho-EIF2S1 (Ser51). Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody
Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody
😃Good
Catalog No. TMAB-01404
Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody is a Rabbit antibody targeting Phospho-EIF2S1 (Ser51). Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody is a Rabbit antibody targeting Phospho-EIF2S1 (Ser51). Anti-Phospho-EIF2S1 (Ser51) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Cow) |
| Verified Activity | 1. Sample: Testis (Mouse) Lysate at 40 μg Primary: Anti-Phospho-eIF2 alpha (Ser51) (TMAB-01404) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 34 kDa 2. Sample: Ovary (Mouse) Lysate at 40 μg Primary: Anti-Phospho-eIF2 alpha (Ser51) (TMAB-01404) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 34 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Phospho-eIF2 alpha (Ser51)) Polyclonal Antibody, Unconjugated (TMAB-01404) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (Phospho-eIF2 alpha (Ser51)) Polyclonal Antibody, Unconjugated (TMAB-01404) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasmic granule. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | eIF2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF2 alpha is phosphorylated at serine 51 (human EIF2 alpha, the equivalent residue in mouse is serine 52) by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important regulatory process in protein synthesis. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human eIF2 alpha around the phosphorylation site of Ser51 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | RNAi,Regulation,Eukaryotic Initiation factors (eIF's) |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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