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Anti-Phospho-CAMKIV (Thr196, 200) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CAMKIV (Thr196, 200). Anti-Phospho-CAMKIV (Thr196, 200) Polyclonal Antibody can be used in WB,IHC,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $217 | 7-10 days | |
100 μL | $318 | 7-10 days |
Description | Anti-Phospho-CAMKIV (Thr196, 200) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CAMKIV (Thr196, 200). Anti-Phospho-CAMKIV (Thr196, 200) Polyclonal Antibody can be used in WB,IHC,IF. |
Synonyms | p-CAMKIV (Thr196, 200), p-CAMKIV (T196, 200), CAMKIV (p-Thr196, 200), CAMKIV (p-T196, 200) |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK4 (Phospho-Thr196/200) Antibody. The picture on the right is blocked with the phospho peptide. 2. Immunofluorescence analysis of HeLa cells, using CaMK4 (Phospho-Thr196/200) Antibody. The picture on the right is blocked with the phospho peptide. 3. Western blot analysis of extracts from K562 cells treated with H2O2 using Phospho-CaMK4 (Thr196/200) antibody TMAC-00539. The lane on the right is treated with the antigen-specific peptide. |
Application | |
Recommended Dose | WB: 1:500-2000; IHC: 1:100-300; IF: 1:200-1000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Appearance | Liquid |
Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Research Background | Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | A synthesized phosphopeptide: human CaMK4 around the phosphorylation site of Thr196/200 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Actual: 60 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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