Anti-Phospho-ASK1 (Thr845) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ASK1 (Thr845). Anti-Phospho-ASK1 (Thr845) Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat

1. Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-phospho-ASK1 (Thr845) antibody (TMAB-01392), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions);
Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
2. Sample:
Lane1: Brain (Rat) Lysate at 30 μg
Lane2: Heart (Rat) Lysate at 30 μg
Primary: Anti-phospho-ASK1 (Thr845) (TMAB-01392) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (secondary antibody) at 1: 3000 dilution;
Predicted band size: 155 kDa
Observed band size: 155 kDa
3. Sample:
Lane 8: Cerebrum (Rat) Lysate at 40 μg
Lane 9: Stomach (Rat) Lysate at 40 μg
Primary: Anti-phospho-ASK1 (Thr845) (TMAB-01392) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 154 kDa
Observed band size: 154 kDa
4. Blank control: U937. Primary Antibody (green line): Rabbit Anti-phospho-ASK1 (Thr845) antibody (TMAB-01392)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-phospho-RelB (Ser551) antibody (TMAB-01392)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 1μg /test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human ASK1 around the phosphorylation site of Thr845

Human

Apoptosis,Hypertrophy,Kinases