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Anti-PGK1 Antibody (1H456)
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Catalog No. TMAC-03177
Alias Primer recognition protein 2, Phosphoglycerate kinase 1, PGKA, PGK1, Cell migration-inducing gene 10 protein
Anti-PGK1 Antibody (1H456) is a Rabbit antibody targeting PGK1. Anti-PGK1 Antibody (1H456) can be used in WB,ICC/IF,IP,FCM.
Anti-PGK1 Antibody (1H456)
Anti-PGK1 Antibody (1H456)
😃Good
Catalog No. TMAC-03177Alias Primer recognition protein 2, Phosphoglycerate kinase 1, PGKA, PGK1, Cell migration-inducing gene 10 protein
Anti-PGK1 Antibody (1H456) is a Rabbit antibody targeting PGK1. Anti-PGK1 Antibody (1H456) can be used in WB,ICC/IF,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Product Introduction
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Chemical Properties
| Description | Anti-PGK1 Antibody (1H456) is a Rabbit antibody targeting PGK1. Anti-PGK1 Antibody (1H456) can be used in WB,ICC/IF,IP,FCM. |
| Synonyms | Primer recognition protein 2, Phosphoglycerate kinase 1, PGKA, PGK1, Cell migration-inducing gene 10 protein |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of PGK1 on different lysates using anti-PGK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: HepG2, Lane 3: MCF-7. 2. ICC staining PGK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. Flow cytometric analysis of Hela cells with PGK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Phosphoglycerate kinases 1/2 (PGK1/2, ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) are somatically expressed, glycolytic enzymes that catalyze the transfer of a phosphoryl group from the acyl phosphate of 1,3-bisphosphoglycerate to ADP, thereby forming ATP and 3-phosphoglycerate. The human PGK gene is interrupted by 10 introns and spans 23 kilobases, and is X chromosome-linked at position Xq21.1, a region implicated in prostate cancer, androgen insensitivity, perineal hypospadias, and other genetic abnormalities. In addition to influencing glycolysis, the PGK1 is secreted by tumor cells and contributes to proliferative angiogenic processes as a disulfide reductase. PGK1 mediated reduction of disulphide bonds in the serine proteinase plasmin initiates the release of the tumor blood vessel inhibitor angiostatin, an event that is critical for blood vessel formation or angiogenesis in tumor expansion and metastasis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 45 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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