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Anti-PDGF R alpha/PDGFRA Antibody (8J71)
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Catalog No. TMAC-03117
Alias RHEPDGFRA, PDGFRA, PDGFR-2, PDGFR2, PDGF R α/PDGFRA Protein, Human, Recombinant (His & Avi), Biotinylated, MGC74795, CD140A
Anti-PDGF R alpha/PDGFRA Antibody (8J71) is a Rabbit antibody targeting PDGF R alpha/PDGFRA. Anti-PDGF R alpha/PDGFRA Antibody (8J71) can be used in WB,ICC,IHC,FCM.
Anti-PDGF R alpha/PDGFRA Antibody (8J71)
Anti-PDGF R alpha/PDGFRA Antibody (8J71)
😃Good
Catalog No. TMAC-03117Alias RHEPDGFRA, PDGFRA, PDGFR-2, PDGFR2, PDGF R α/PDGFRA Protein, Human, Recombinant (His & Avi), Biotinylated, MGC74795, CD140A
Anti-PDGF R alpha/PDGFRA Antibody (8J71) is a Rabbit antibody targeting PDGF R alpha/PDGFRA. Anti-PDGF R alpha/PDGFRA Antibody (8J71) can be used in WB,ICC,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-PDGF R alpha/PDGFRA Antibody (8J71) is a Rabbit antibody targeting PDGF R alpha/PDGFRA. Anti-PDGF R alpha/PDGFRA Antibody (8J71) can be used in WB,ICC,IHC,FCM. |
| Synonyms | RHEPDGFRA, PDGFRA, PDGFR-2, PDGFR2, PDGF R α/PDGFRA Protein, Human, Recombinant (His & Avi), Biotinylated, MGC74795, CD140A |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of PDGFR alpha on NIH/3T3 cells lysates using anti-PDGFR alpha antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin. 5. ICC staining PDGFR alpha in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining PDGFR alpha in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with PDGFR alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-α and -β, which are endowed with protein tyrosine kinase domains. PDGFR-α can bind to both A and B subunits of PDGF, while PDGFR-β can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two α receptors, PDGF-AB induces dimerization of αα and αβ and PDGF-BB induces the formation of three types of dimers, αα, αβ and ββ. Translocation of the PDGFR-β gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 123 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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