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Anti-NF-kB p65 Antibody (9V807)
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Catalog No. TMAB-01228
Alias v-rel avian reticuloendotheliosis viral oncogene homolog A, p65, NFKB3
Anti-NF-kB p65 Antibody (9V807) is a Mouse antibody targeting NF-kB p65. Anti-NF-kB p65 Antibody (9V807) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-NF-kB p65 Antibody (9V807)
Anti-NF-kB p65 Antibody (9V807)
😃Good
Catalog No. TMAB-01228Alias v-rel avian reticuloendotheliosis viral oncogene homolog A, p65, NFKB3
Anti-NF-kB p65 Antibody (9V807) is a Mouse antibody targeting NF-kB p65. Anti-NF-kB p65 Antibody (9V807) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-NF-kB p65 Antibody (9V807) is a Mouse antibody targeting NF-kB p65. Anti-NF-kB p65 Antibody (9V807) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Alias | v-rel avian reticuloendotheliosis viral oncogene homolog A, p65, NFKB3 |
| Ig Type | IgG1 |
| Clone | 9V807 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: RELA-Ko (Human) Cell Lysate at 30 μg Hela (Human) Cell Lysate at 30 μg Primary: Anti-NFKB p65 (RELA) (TMAB-01228) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 65 kDa Observed band size: 65 kDa 2. 25 μg total protein per Lane of various lysates probed with NFKB p65 monoclonal antibody, unconjugated (TMAB-01228) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 3. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (NFKB p65) monoclonal Antibody, unconjugated (TMAB-01228) 1:50, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (TMAB-01228) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (TMAB-01228) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Spleen；Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (ascites of TMAB-01228) 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Tonsil;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (ascites of TMAB-01228) 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (TMAB-01228) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (TMAB-01228) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (TMAB-01228) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC-P: 1:200-1500; IHC-Fr: 1:200-1500; ICC/IF: 1:50; IF: 1:200-1500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Nucleus. Cytoplasm. Note=Colocalized with DDX1 in the nucleus upon TNF-alpha induction. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]. |
| Immunogen | Recombinant Protein: human NFKB p65 Protein |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | RelA (p65),NFkB pathway,Inflammatory mediators,RelA (p65),NFkB pathway,Obesity,Host Immune Response,Host Virus Interaction,Alzheimer's disease,NFkB Pathway |
| Molecular Weight | Theoretical: 61 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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