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Anti-NET1 Polyclonal Antibody

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Catalog No. TMAB-01216

Anti-NET1 Polyclonal Antibody is a Rabbit antibody targeting NET1. Anti-NET1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-NET1 Polyclonal Antibody

Anti-NET1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01216
Anti-NET1 Polyclonal Antibody is a Rabbit antibody targeting NET1. Anti-NET1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$374 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-NET1 Polyclonal Antibody is a Rabbit antibody targeting NET1. Anti-NET1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NET1 Polyclonal Antibody, Unconjugated (TMAB-01216) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Jurkat (Human) Cell Lysate at 30 μg
Skin (Mouse) Lysate at 40 μg
Skin (Rat) Lysate at 40 μg
Primary: Anti-NET1 (TMAB-01216) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
3. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Rat Cerebellum tissue lysates
Lane 6: Rat Stomach tissue lysates
Lane 7: Human K562 cell lysates
Lane 8: Human A431 cell lysates
Lane 9: Human HepG2 cell lysates
Lane 10: Human MCF-7 cell lysates
Primary: Anti-NET1 (TMAB-01216) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 62 kDa
4. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (NET1) polyclonal Antibody, Unconjugated (TMAB-01216) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:25; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm (By similarity). Nucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NET1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Ras family,Other factors,Ras Family
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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