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Anti-N Cadherin Polyclonal Antibody 2 is a Rabbit antibody targeting N Cadherin. Anti-N Cadherin Polyclonal Antibody 2 can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Antibody Type: Rabbit Polyclonal Application: WB Reactivity: Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit,Sheep) |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit,Sheep) |
Verified Activity | 1. Sample: Lane 1: Heart (Mouse) Lysate at 40 μg Lane 2: A549 (Human) Cell Lysate at 30 μg Primary: Anti-N-Cadherin (TMAB-01199) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 130 kDa 2. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-N-cadherin (TMAB-01199) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kDa Observed band size: 105/125 kDa 3. Sample: Lane 1: Testis (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Mouse) Lysate at 40 μg Lane 3: Testis (Rat) Lysate at 40 μg Lane 4: Cerebellum (Rat) Lysate at 40 μg Lane 5: Panc-1 (Human) Cell Lysate at 30 μg Lane 6: A549 (Human) Cell Lysate at 30 μg Lane 7: SH-SY5Y (Human) Cell Lysate at 30 μg Lane 8: U251 (Human) Cell Lysate at 30 μg Primary: Anti-N-cadherin (TMAB-01199) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 130 kDa 4. Sample: Lane 1: A549 (Human) Cell Lysate at 30 μg Lane 2: SH-SY5Y (Human) Cell Lysate at 30 μg Lane 3: U251 (Human) Cell Lysate at 30 μg Lane 4: MCF-7 (Human) Cell Lysate at 30 μg Primary: Anti-N-cadherin (TMAB-01199) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 130 kDa |
Application | WB |
Recommended Dose | WB: 1:500-2000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. |
Immunogen | KLH conjugated synthetic peptide: human N-Cadherin |
Antigen Species | Human |
Gene ID | 1000 |
Uniprot ID | |
Biology Area | Cadherins,Cadherins,Surface Molecules |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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