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Anti-MARCH7 Polyclonal Antibody

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Catalog No. TMAB-01104

Anti-MARCH7 Polyclonal Antibody is a Rabbit antibody targeting MARCH7. Anti-MARCH7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-MARCH7 Polyclonal Antibody

Anti-MARCH7 Polyclonal Antibody

😃Good
Catalog No. TMAB-01104
Anti-MARCH7 Polyclonal Antibody is a Rabbit antibody targeting MARCH7. Anti-MARCH7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$374 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-MARCH7 Polyclonal Antibody is a Rabbit antibody targeting MARCH7. Anti-MARCH7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (TMAB-01104) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (TMAB-01104) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 μg
Lane 2: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-MARCH7 (TMAB-01104) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 78/74 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundAxotrophin is a stem cell gene that encodes a protein which is involved in T lymphocyte regulation (especially in regulating the proliferation) and leukemia inhibitory factor (LIF) release. LIF is a neuropoietic cytokine that is important for stem cell regulation and thymocyte stimulation. Both Axotrophin and LIF are linked to transplantation intolerance. Axotrophin is also involved in corpus callosum differentiation and may play a role in glial cell line-derived neurotrophic factor (GDNF)-dependent sensory neuron survival in the substantia gelatinosa of the adult spinal cord. Axotrophin is primarily expressed in the hippocampus, cortex, purkinje and granule cells of the cerebellum.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human MARCH7/Axotrophin
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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