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Anti-M13 Bacteriophage Antibody-PE (4Z973)
🥰Excellent
Catalog No. TMAY-02172P
Anti-M13 Bacteriophage Antibody-PE (4Z973) is a PE-conjugated Mouse antibody targeting M13 Bacteriophage. Anti-M13 Bacteriophage Antibody-PE (4Z973) can be used in FCM.
Anti-M13 Bacteriophage Antibody-PE (4Z973)
Anti-M13 Bacteriophage Antibody-PE (4Z973)
🥰Excellent
Catalog No. TMAY-02172P
Anti-M13 Bacteriophage Antibody-PE (4Z973) is a PE-conjugated Mouse antibody targeting M13 Bacteriophage. Anti-M13 Bacteriophage Antibody-PE (4Z973) can be used in FCM.
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Product Introduction
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Chemical Properties
| Description | Anti-M13 Bacteriophage Antibody-PE (4Z973) is a PE-conjugated Mouse antibody targeting M13 Bacteriophage. Anti-M13 Bacteriophage Antibody-PE (4Z973) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 4Z973 |
| Reactivity | other |
| Verified Activity | 4-1BB-transfected CHO-K1 cells were incubated with M13 phage particles for 20 minutes, then detected with 10 μL phage M13-PE Ab. The cell line stained with a 4-1BB specific M13 phage antibody (filied profile). Compared to an irrelevant phage antibody (open profile).The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM |
| Recommended Dose | 10 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This product is a recombinant monoclonal antibody expressed from HEK293 cells. Then conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Research Background | M13 is a filamentous bacteriophage composed of circular single-stranded DNA (ssDNA) which is 647 nucleotides long encapsulated in approximately 27 copies of the major coat protein P8 and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however, the infection causes turbid plaques in E. coli. It is a non-lytic virus. However, a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 5 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 27 copies of p8 to make the coat about 9 nm long. The coat's dimensions are flexible though and the number of p8 copies adjusts to accommodate the size of the single-stranded genome it packages. The general stages to a viral life cycle are infection, replication of the viral genome, assembly of new viral particles, and then release of the progeny particles from the host. Filamentous phage uses a bacterial structure known as the F pilus to infect E. coli, with the M13 p3 tip contacting the TolA protein on the bacterial pilus. The phage genome is then transferred to the cytoplasm of the bacterial cell where resident proteins convert the single-stranded DNA genome to a double-stranded replicative form. |
| Conjucates | PE |
| Others Formats | Unconjugated/Biotin/FITC/HRP |
| Antibody Types Available | 5 |
| Immunogen | M13 Bacteriophage |
| Antigen Species | other |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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