Anti-LDLR Polyclonal Antibody is a Rabbit antibody targeting LDLR. Anti-LDLR Polyclonal Antibody can be used in WB,ICC/IF,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit,GuineaPig)

1. Sample: Human colon Lysate at 45 μg;
Primary: Anti-LDL receptor (TMAB-01057) at 1:300 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 5000 dilution;
Predicted band size: 92 kDa
Observed band size: 92 kDa
2. Sample:
K562 (Human) Cell Lysate at 30 μg
Raw264.7 (Mouse) Cell Lysate at 30 μg
HepG2 (Human) Cell Lysate at 30 μg
SW480 (Human) Cell Lysate at 30 μg
Primary: Anti-LDL receptor (TMAB-01057) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 120 kDa
3. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (TMAB-01057)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
4. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (LDL receptor) polyclonal Antibody, Unconjugated (TMAB-01057) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
5. Blank control (black line): HepG2 (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (green line): Rabbit Anti-LDLreceptor antibody (TMAB-01057);
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1 μg/test.
6. Blank control: A431. Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (TMAB-01057), Dilution: 1 μg/10^6 cells.
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 μg/test.
Protocol
A431 cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,ICC/IF,FCM

WB: 1:500-2000; ICC/IF: 1:100; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human LDL-R

Human

Metabolism of lipids and lipoproteins,Lipoprotein metabolism,Cholesterol Metabolism,Cholesterol Metabolism,Lipid metabolism,Lipoprotein metabolism,Cancer,Heart disease,Lipid metabolism,Lipid Messengers