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Anti-LAMP1 Polyclonal Antibody is a Rabbit antibody targeting LAMP1. Anti-LAMP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-LAMP1 Polyclonal Antibody is a Rabbit antibody targeting LAMP1. Anti-LAMP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
Ig Type | IgG |
Reactivity | Human |
Verified Activity | 1. Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (LAMP1) Polyclonal Antibody, Unconjugated (TMAB-01046) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (5 μg/ml, blue) was used to stain the cell nucleus. 2. Sample: Lane 1: Human HT-1080 cell lysates Lane 2: Human HeLa cell lysates Lane 3: Human JAR cell lysates Primary: Anti-LAMP1 (TMAB-01046) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Observed band size: 95 kDa 3. Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-LAMP1 antibody (TMAB-01046) Dilution: 2 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 4. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (LAMP1) polyclonal Antibody, unconjugated (TMAB-01046) 1:200, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. 5. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (TMAB-01046) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (TMAB-01046) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (TMAB-01046) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (TMAB-01046) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (TMAB-01046) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (TMAB-01046) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (TMAB-01046) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (TMAB-01046) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 13. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (TMAB-01046) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 14. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (TMAB-01046) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 15. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Polyclonal Antibody, Unconjugated (TMAB-01046) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM |
Recommended Dose | WB: 1:500-1:2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; FCM: 2ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation. |
Immunogen | KLH conjugated synthetic peptide: human LAMP-1 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Signal Transduction,Autophagy,Autophagy and mitophagy,SARS Coronavirus,Growth Cone,Soma marker,Neurogenesis,lamp1,Organelle Proteins,B Lymphocytic Lineage,T Lymphocytic Lineage,Monocytic Lineage,Lysosome |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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