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Anti-IL-1 beta Polyclonal Antibody
😃Good
Catalog No. TMAB-00932
Anti-IL-1 beta Polyclonal Antibody is a Rabbit antibody targeting IL-1 beta. Anti-IL-1 beta Polyclonal Antibody can be used in WB.
Anti-IL-1 beta Polyclonal Antibody
Anti-IL-1 beta Polyclonal Antibody
😃Good
Catalog No. TMAB-00932
Anti-IL-1 beta Polyclonal Antibody is a Rabbit antibody targeting IL-1 beta. Anti-IL-1 beta Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB Reactivity: Mouse,Human |
| Ig Type | IgG |
| Reactivity | Mouse,Human |
| Verified Activity | 1. Sample: Lane 1: Normal Mouse Raw264.7 cell lysates Lane 2: Raw264.7 treated with 100ng/ml lipopolysaccharide (LPS) for 6h, then with 300ng/ml Brefeldin A added after 3 hours Primary: Anti-IL-1 Beta (TMAB-00932) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/32 kDa Observed band size: 35 kDa 2. THP-1 (H) cells were treated with or without TPA (80nM) for 24 h,then treated with (100ng/ml) lipopolysaccharide (LPS) for 6 h, then with (300ng/ml) Brefeldin A added after 3 h, 25 μg total protein per Lane of cell lysates probed with IL-1 Beta polyclonal antibody, unconjugated (TMAB-00932) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| Application | WB |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human IL-1beta |
| Antigen Species | Human |
| Gene ID | 3553 |
| Uniprot ID | |
| Biology Area | Inflammatory mediators,Interleukins,Obesity,SARS Coronavirus |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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