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Anti-ICAM-1/CD54 Antibody-FITC (4H175)
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Catalog No. TMAY-00663F
Alias P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2
Anti-ICAM-1/CD54 Antibody-FITC (4H175) is a FITC-conjugated Mouse antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody-FITC (4H175) can be used in FCM.
Anti-ICAM-1/CD54 Antibody-FITC (4H175)
Anti-ICAM-1/CD54 Antibody-FITC (4H175)
😃Good
Catalog No. TMAY-00663FAlias P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2
Anti-ICAM-1/CD54 Antibody-FITC (4H175) is a FITC-conjugated Mouse antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody-FITC (4H175) can be used in FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 T | $82 | 7-10 days | |
| 100 T | $174 | 7-10 days |
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| Description | Anti-ICAM-1/CD54 Antibody-FITC (4H175) is a FITC-conjugated Mouse antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody-FITC (4H175) can be used in FCM. |
| Alias | P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 4H175 |
| Reactivity | Human |
| Verified Activity | 1. Profile of anti-ICAM1(CD54) reactivity on Daudi cells analyzed by flow cytometry. 2. Flow cytometric analysis of Human ICAM1 (CD54) in HUVEC cells. HUVEC cells (Human umbilical vein endothelial cells) were treated with TNF-α (50 ng/ml,18 hours). The cells were harvested and stained with FITC Mouse anti-Human ICAM1. |
| Application | FCM |
| Recommended Dose | 10 μl/Test, 0.1 mg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM-1 / CD54 (rh ICAM-1 / CD54; TMPY-01142; NP_000192.2; Met 1-Glu 480) and conjugated with FITC under optimum conditions, the unreacted FITC was removed. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| Research Background | Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity. |
| Conjucates | FITC |
| Others Formats | Unconjugated/APC/PerCP/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Human ICAM-1 protein (TMPY-01142) |
| Antigen Species | Human |
| Biology Area | Neuroinflammation |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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