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Anti-HSP90AB1 Antibody (4Y129)
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Catalog No. TMAC-02050
Alias HSP84)
Anti-HSP90AB1 Antibody (4Y129) is a Rabbit antibody targeting HSP90AB1. Anti-HSP90AB1 Antibody (4Y129) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-HSP90AB1 Antibody (4Y129)
Anti-HSP90AB1 Antibody (4Y129)
😃Good
Catalog No. TMAC-02050Alias HSP84)
Anti-HSP90AB1 Antibody (4Y129) is a Rabbit antibody targeting HSP90AB1. Anti-HSP90AB1 Antibody (4Y129) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-HSP90AB1 Antibody (4Y129) is a Rabbit antibody targeting HSP90AB1. Anti-HSP90AB1 Antibody (4Y129) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | HSP84) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Hsp90 beta on different lysates using anti-Hsp90 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: K562. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsp90 beta antibody. Counter stained with hematoxylin. 7. ICC staining Hsp90 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Hsp90 beta in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining Hsp90 beta in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of Jurkat cells with Hsp90 beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The heat shock response was first described for Drosophila salivary gland cells and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial chicken and mammalian cells, and have been subsequently studied in other organisms. A series of proteins including HSP 90, HSP 70, HSP 20-30 and ubiquitin are induced by insults such as temperature shock, chemicals and other environmental stress. A major function of HSP 90 and other HSPs is to act as molecular chaperones. HSP 90 forms a complex with glucocorticoid receptor (GR), rendering the non ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP 56 or Cyclophilin D, forming an aporeceptor comiplex. HSP 90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an apo-receptor complex with estrogen and androgen receptors. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 83 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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