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Anti-HIF1AN Antibody (9B616)
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Catalog No. TMAC-01820
Anti-HIF1AN Antibody (9B616) is a Rabbit antibody targeting HIF1AN. Anti-HIF1AN Antibody (9B616) can be used in FCM,ICC,IHC,WB.
Anti-HIF1AN Antibody (9B616)
Anti-HIF1AN Antibody (9B616)
😃Good
Catalog No. TMAC-01820
Anti-HIF1AN Antibody (9B616) is a Rabbit antibody targeting HIF1AN. Anti-HIF1AN Antibody (9B616) can be used in FCM,ICC,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-HIF1AN Antibody (9B616) is a Rabbit antibody targeting HIF1AN. Anti-HIF1AN Antibody (9B616) can be used in FCM,ICC,IHC,WB. |
| Ig Type | IgG |
| Clone | 9B616 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of HIF1AN on A549 and Jurkat cell lysates using anti-HIF1AN antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-HIF1AN antibody. Counter stained with hematoxylin. 3. ICC staining HIF1AN in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining HIF1AN in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of LOVO cells with HIF1AN antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.      |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1α. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the β-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1α. This hydroxylation event blocks the association of HIF-1α with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1α. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm. The structure of FIH-1 includes a jellyroll-like β-barrel containing ferrous-binding triad residues. The gene encoding human FIH-1 maps to chromosome 10q24. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: human HIF1AN aa 1-200 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 40 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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