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Anti-GRP75 Polyclonal Antibody

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Catalog No. TMAB-00816
Alias Stress 70 protein mitochondrial precursor, Stress 70 protein mitochondrial, Peptide binding protein 74, PBP74, P66 MOT, p66 mortalin, Mthsp70, MOT2, MOT 2, MOT, Mortalin2, Mortalin perinuclear, Mortalin 2, Mortalin, MGC4500, HSPA9B, Hspa9a, HSPA9, Hsp74a, Hsp74, Hsc74, Heat shock protein cognate 74, Heat shock protein A, Heat shock protein 74 kDa A, Heat shock 70kDa protein 9B, Heat shock 70kD protein 9, Grp 75, Glucose Regulated Protein, G protein-coupled receptor75, CSA, 75 kDa glucose-regulated protein

Anti-GRP75 Polyclonal Antibody is a Rabbit antibody targeting GRP75. Anti-GRP75 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-GRP75 Polyclonal Antibody

Anti-GRP75 Polyclonal Antibody

😃Good
Catalog No. TMAB-00816Alias Stress 70 protein mitochondrial precursor, Stress 70 protein mitochondrial, Peptide binding protein 74, PBP74, P66 MOT, p66 mortalin, Mthsp70, MOT2, MOT 2, MOT, Mortalin2, Mortalin perinuclear, Mortalin 2, Mortalin, MGC4500, HSPA9B, Hspa9a, HSPA9, Hsp74a, Hsp74, Hsc74, Heat shock protein cognate 74, Heat shock protein A, Heat shock protein 74 kDa A, Heat shock 70kDa protein 9B, Heat shock 70kD protein 9, Grp 75, Glucose Regulated Protein, G protein-coupled receptor75, CSA, 75 kDa glucose-regulated protein
Anti-GRP75 Polyclonal Antibody is a Rabbit antibody targeting GRP75. Anti-GRP75 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-GRP75 Polyclonal Antibody is a Rabbit antibody targeting GRP75. Anti-GRP75 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasStress 70 protein mitochondrial precursor, Stress 70 protein mitochondrial, Peptide binding protein 74, PBP74, P66 MOT, p66 mortalin, Mthsp70, MOT2, MOT 2, MOT, Mortalin2, Mortalin perinuclear, Mortalin 2, Mortalin, MGC4500, HSPA9B, Hspa9a, HSPA9, Hsp74a, Hsp74, Hsc74, Heat shock protein cognate 74, Heat shock protein A, Heat shock protein 74 kDa A, Heat shock 70kDa protein 9B, Heat shock 70kD protein 9, Grp 75, Glucose Regulated Protein, G protein-coupled receptor75, CSA, 75 kDa glucose-regulated protein
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Rat Liver tissue lysates
Lane 5: Rat Testis tissue lysates
Lane 6: Rat Cerebrum tissue lysates
Lane 7: Rat Pancreas tissue lysates
Lane 8: Human K562 cell lysates
Lane 9: Human MCF-7 cell lysates
Lane 10: Human Jurkat cell lysates
Lane 11: Human HeLa cell lysates
Lane 12: Human HepG2 cell lysates
Primary: Anti-GRP75 (TMAB-00816) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 72 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (TMAB-00816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (TMAB-00816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (TMAB-00816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (TMAB-00816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (TMAB-00816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (TMAB-00816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMitochondrion. Nucleus, nucleolus.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GRP75
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cell division,Tumor biomarkers,Mitochondrial Biogenesis,Mitochondrial markers,Alzheimer's disease,Parkinson's disease,Mitochondrial,Heat Shock Proteins
Chemical Properties
Molecular WeightTheoretical: 70 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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