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Anti-GRP75 Polyclonal Antibody is a Rabbit antibody targeting GRP75. Anti-GRP75 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-GRP75 Polyclonal Antibody is a Rabbit antibody targeting GRP75. Anti-GRP75 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | Stress 70 protein mitochondrial precursor, Stress 70 protein mitochondrial, Peptide binding protein 74, PBP74, P66 MOT, p66 mortalin, Mthsp70, MOT2, MOT 2, MOT, Mortalin2, Mortalin perinuclear, Mortalin 2, Mortalin, MGC4500, HSPA9B, Hspa9a, HSPA9, Hsp74a, Hsp74, Hsc74, Heat shock protein cognate 74, Heat shock protein A, Heat shock protein 74 kDa A, Heat shock 70kDa protein 9B, Heat shock 70kD protein 9, Grp 75, Glucose Regulated Protein, G protein-coupled receptor75, CSA, 75 kDa glucose-regulated protein |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit) |
Verified Activity | 1. Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Mouse Testis tissue lysates Lane 3: Mouse Cerebrum tissue lysates Lane 4: Rat Liver tissue lysates Lane 5: Rat Testis tissue lysates Lane 6: Rat Cerebrum tissue lysates Lane 7: Rat Pancreas tissue lysates Lane 8: Human K562 cell lysates Lane 9: Human MCF-7 cell lysates Lane 10: Human Jurkat cell lysates Lane 11: Human HeLa cell lysates Lane 12: Human HepG2 cell lysates Primary: Anti-GRP75 (TMAB-00816) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kDa Observed band size: 72 kDa 2. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (TMAB-00816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (TMAB-00816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (TMAB-00816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (TMAB-00816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (TMAB-00816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (GRP75) Polyclonal Antibody, Unconjugated (TMAB-00816) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
Application | |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Mitochondrion. Nucleus, nucleolus. |
Construction | Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2. |
Immunogen | KLH conjugated synthetic peptide: human GRP75 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Cell division,Tumor biomarkers,Mitochondrial Biogenesis,Mitochondrial markers,Alzheimer's disease,Parkinson's disease,Mitochondrial,Heat Shock Proteins |
Molecular Weight | Theoretical: 70 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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