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Anti-GPR84 Polyclonal Antibody
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Catalog No. TMAB-00799
Alias Inflammation-related G-protein coupled receptor EX33, G-protein coupled receptor 84, GPR84, EX33
Anti-GPR84 Polyclonal Antibody is a Rabbit antibody targeting GPR84. Anti-GPR84 Polyclonal Antibody can be used in WB.
Anti-GPR84 Polyclonal Antibody
Anti-GPR84 Polyclonal Antibody
😃Good
Catalog No. TMAB-00799Alias Inflammation-related G-protein coupled receptor EX33, G-protein coupled receptor 84, GPR84, EX33
Anti-GPR84 Polyclonal Antibody is a Rabbit antibody targeting GPR84. Anti-GPR84 Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-GPR84 Polyclonal Antibody is a Rabbit antibody targeting GPR84. Anti-GPR84 Polyclonal Antibody can be used in WB. |
| Synonyms | Inflammation-related G-protein coupled receptor EX33, G-protein coupled receptor 84, GPR84, EX33 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Cow,Horse,Sheep,Monkey) |
| Verified Activity | 1. Sample: bone (Rat) Lysate at 40 μg Primary: Anti-GPR84 (TMAB-00799) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa 2. Sample: SP2/0 (mouse) cell Lysate at 40 μg Lung (mouse) Lysate at 40 μg HL-60 (human) cell Lysate at 40 μg Muscle (mouse) Lysate at 40 μg Primary: Anti-GPR84 (TMAB-00799) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa 3. Sample: Lane 1: Blood cell (Mouse) Lysate at 40 μg Lane 2: Lung (Mouse) Lysate at 40 μg Lane 3: Bone (Rat) Lysate at 40 μg Lane 4: Lung (Rat) Lysate at 40 μg Lane 5: Cerebrum (Rat) Lysate at 40 μg Lane 6: Cerebellum (Rat) Lysate at 40 μg Lane 7: U937 (Human) Cell Lysate at 30 μg Lane 8: U87MG (Human) Cell Lysate at 30 μg Lane 9: THP-1 (Human) Cell Lysate at 30 μg Primary: Anti-GPR84 (TMAB-00799) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 44 kDa    |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Tissue Specificity | Expressed predominantly in hematopoietic tissues. High levels detected in the bone marrow and lower levels in the peripheral leukocytes and lung. Also expressed in brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta and intestine. Within |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors and heptahelical receptors, are a protein family which interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers such as diacylglycerol, cyclic AMP, inositol phosphates, and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. G protein receptor 84 (GPR84), a member of the GCPR 1 family, is an orphan GCPR expressed in bone marrow, brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta, intestine, lung and peripheral blood leukocytes. In activated T cells, GPR84 regulates early interleukin-4 (IL-4) gene expression |
| Immunogen | KLH conjugated synthetic peptide: human GPCR EX33/GPR84 |
| Antigen Species | Human |
| Gene Name | GPR84 |
| Gene ID | |
| Protein Name | G-protein coupled receptor 84 |
| Uniprot ID | |
| Biology Area | GPCR |
| Function | Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system. |
| Molecular Weight | Theoretical: 44 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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