Anti-GFAP Antibody (6C642) is a Mouse antibody targeting GFAP. Anti-GFAP Antibody (6C642) can be used in WB,IHC,IF.

6C642

Human,Mouse,Rat

1. Western blot analysis of lysates from 1) Rat Brain Tissue, 2)HeLa , 3)A431, 4) PC12 cells, (Green) primary antibody was diluted at 1:1000, 4°Cover night, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Tubulin β Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.
2. Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,GFAP Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,2 0min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
3. Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,GFAP Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3,Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
4. Immunofluorescence analysis of Rat-brain tissue. 1,GFAP Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
5. Immunofluorescence analysis of Mouse-brain tissue. 1,GFAP Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
6. Immunofluorescence analysis of Hela cell. 1,AR Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GFAP Monoclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

WB: 1:2000-5000; IHC: 1:50-300; IF: 1:100-200

Monoclonal

Unconjugated