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Anti-GCLM Antibody (9X353) (Synonyms: GSH0_HUMAN, GSC light chain, Glutamate--cysteine ligase regulatory subun...)
Catalog No. TMAC-01635 Copy Product Info
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Anti-GCLM Antibody (9X353) is a Rabbit antibody targeting GCLM. Anti-GCLM Antibody (9X353) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-GCLM Antibody (9X353)
Copy Product Info🥰Excellent
Catalog No. TMAC-01635
Synonyms GSH0_HUMAN, GSC light chain, Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, Glutamate cysteine ligase regulatory subunit, GLCLR, GCS light chain, GCLM, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit, Gamma ECS regulatory subunit
Anti-GCLM Antibody (9X353) is a Rabbit antibody targeting GCLM. Anti-GCLM Antibody (9X353) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-GCLM Antibody
(9X353)
(9X353)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $297 | 7-10 days | 7-10 days | |
| 100 μL | $497 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
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Chemical Properties
Stability & Storage
| Description | Anti-GCLM Antibody (9X353) is a Rabbit antibody targeting GCLM. Anti-GCLM Antibody (9X353) can be used in FCM,ICC/IF,IHC,IP,WB. |
| Synonyms | GSH0_HUMAN, GSC light chain, Glutamate--cysteine ligase regulatory subunit, Glutamate--cysteine ligase modifier subunit, Glutamate cysteine ligase regulatory subunit, GLCLR, GCS light chain, GCLM, Gamma-glutamylcysteine synthetase regulatory subunit, Gamma-ECS regulatory subunit, Gamma ECS regulatory subunit |
| Ig Type | IgG |
| Clone | 9X353 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of GCLM on different cell lysates using anti-GCLM antibody at 1/500 dilution. Positive control: Lane 1: A431, Lane 2: PC-12, Lane 3: NIH-3T3. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GCLM antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GCLM antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-GCLM antibody. Counter stained with hematoxylin. 5. ICC staining GCLM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining GCLM in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining GCLM in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with GCLM antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).         |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-100; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Gamma-glutamylcysteine synthetase (γ-GCS) is the rate limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. γ-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human gamma-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human γ-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of γ-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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