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Anti-FOXO3 Antibody (6R502) (Synonyms: MGC31925, MGC12739, FOXO3A, FOX O3A, Forkhead in rhabdomyosarcoma-lik...)

Catalog No. TMAC-01551 Copy Product Info
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Anti-FOXO3 Antibody (6R502) is a Rabbit antibody targeting FOXO3. Anti-FOXO3 Antibody (6R502) can be used in ICC/IF,WB.

Anti-FOXO3 Antibody (6R502)

Copy Product Info
🥰Excellent
Catalog No. TMAC-01551
Synonyms MGC31925, MGC12739, FOXO3A, FOX O3A, Forkhead in rhabdomyosarcoma-like 1, Forkhead homolog (rhabdomyosarcoma) like 1, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1, Forkhead box O3A, Forkhead box O3, Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1, FKHRL1P2, FKHRL1, FKHRL 1, FKHR2, DKFZp781A0677, AF6q21

Anti-FOXO3 Antibody (6R502) is a Rabbit antibody targeting FOXO3. Anti-FOXO3 Antibody (6R502) can be used in ICC/IF,WB.

Anti-FOXO3 Antibody
(6R502)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2987-10 days7-10 days
100 μL$4977-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-FOXO3 Antibody (6R502) is a Rabbit antibody targeting FOXO3. Anti-FOXO3 Antibody (6R502) can be used in ICC/IF,WB.
SynonymsMGC31925, MGC12739, FOXO3A, FOX O3A, Forkhead in rhabdomyosarcoma-like 1, Forkhead homolog (rhabdomyosarcoma) like 1, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1, Forkhead box O3A, Forkhead box O3, Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1, FKHRL1P2, FKHRL1, FKHRL 1, FKHR2, DKFZp781A0677, AF6q21
Ig Type
IgG
Clone
6R502
Reactivity
Human
Verified Activity
1. Western blot analysis of FOXO3A on different lysates using anti- FOXO3A antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3, Lane 2: PC-12, Lane 3: MCF-7.
2. ICC staining FOXO3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining FOXO3A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining FOXO3A in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
verifiedActivityverifiedActivityverifiedActivityverifiedActivity
Application
Recommended Dose
WB: 1:1000-5000; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundFKHRL1 (forkhead in rhabdomyosarcoma-like 1), also known as FOXO3 (forkheadbox O3) or FOXO3A, is a 673 amino acid transcriptional activator thatbelongs to the FKHR subfamily of forkhead transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 at Threonine 32 and Serine 253. Phosphorylation by Akt1 negatively regulates FKHRL1 by promoting its export from the nucleus. Phosphorylated FKHRL1 associates with 14-3-3 proteins and this complex is retained in the cytoplasm. Growth factor withdrawal stimulates FKHRL1 dephosphorylation and nuclear translocation, leading to FKHR-induced gene-specific transcriptional activation. Within the nucleus, dephosphorylated FKHRL1 triggers apoptosis by inducing the expression of genes that are critical for cell death.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 71 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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