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Anti-Fas/CD95 Polyclonal Antibody is a Rabbit antibody targeting Fas/CD95. Anti-Fas/CD95 Polyclonal Antibody can be used in WB,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-Fas/CD95 Polyclonal Antibody is a Rabbit antibody targeting Fas/CD95. Anti-Fas/CD95 Polyclonal Antibody can be used in WB,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Pig) |
Verified Activity | 1. Sample: Lane 1: Thymus (Mouse) Lysate at 40 μg Lane 2: Thymus (Rat) Lysate at 40 μg Lane 3: HepG2 (Human) Cell Lysate at 30 μg Lane 4: Raji (Human) Cell Lysate at 30 μg Lane 5: HT1080 (Human) Cell Lysate at 30 μg Lane 6: A431 (Human) Cell Lysate at 30 μg Primary: Anti-CD95/FAS (TMAB-00656) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45/52 kDa Observed band size: 52 kDa 2. Sample: serum (Rat) at 40 μg plasma (Rat) at 40 μg Primary: Anti-CD95 (TMAB-00656) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 42 kDa 3. Blank control (blue): Mouse Kidney (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-CD95/FAS antibody (TMAB-00656,Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 4. Blank control: Raji. Primary Antibody (green line): Rabbit Anti-CD95/FAS antibody (TMAB-00656) Dilution: 2 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | WB,FCM |
Recommended Dose | WB: 1:500-2000; FCM: 2μg/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2, 3, 4, 5, 6: Secreted. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011] |
Immunogen | KLH conjugated synthetic peptide: rat FAS |
Antigen Species | Rat |
Gene ID | |
Uniprot ID | |
Biology Area | Death Receptors,Cytokines,TNF Superfamily |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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