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Anti-Elastin Polyclonal Antibody
😃Good
Catalog No. TMAB-00604
Alias Tropoelastin, Eln, Elastin
Anti-Elastin Polyclonal Antibody is a Rabbit antibody targeting Elastin. Anti-Elastin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Elastin Polyclonal Antibody
Anti-Elastin Polyclonal Antibody
😃Good
Catalog No. TMAB-00604Alias Tropoelastin, Eln, Elastin
Anti-Elastin Polyclonal Antibody is a Rabbit antibody targeting Elastin. Anti-Elastin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Elastin Polyclonal Antibody is a Rabbit antibody targeting Elastin. Anti-Elastin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Tropoelastin, Eln, Elastin |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Elastin) Polyclonal Antibody, Unconjugated (TMAB-00604) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Elastin Polyclonal Antibody, Unconjugated (TMAB-00604) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Elastin Polyclonal Antibody, Unconjugated (TMAB-00604) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Sample: Lane 1: Lung (Mouse) Lysate at 40 μg Lane 2: Liver (Mouse) Lysate at 40 μg Lane 3: Uterus (Rat) Lysate at 40 μg Lane 4: Liver (Rat) Lysate at 40 μg Primary: Anti-Elastin (TMAB-00604) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50-80 kDa Observed band size: 75 kDa 5. Sample: Lung (Mouse) Lysate at 40 μg Primary: Anti-Elastin (TMAB-00604) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa 6. Sample: Uterus (Mouse) Lysate at 40 μg Primary: Anti-Elastin (TMAB-00604) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. Note=Extracellular matrix of elastic fibers. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a protein that is one of the two components of elastic fibers. Elastic fibers comprise part of the extracellular matrix and confer elasticity to organs and tissues including the heart, skin, lungs, ligaments, and blood vessels. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Degradation products of the encoded protein, known as elastin-derived peptides or elastokines, bind the elastin receptor complex and other receptors and stimulate migration and proliferation of monocytes and skin fibroblasts. Elastokines can also contribute to cancer progression. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. [provided by RefSeq, Aug 2017]. |
| Immunogen | KLH conjugated synthetic peptide: human Elastin |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID |
| Molecular Weight | Theoretical: 68 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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