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Anti-Doublecortin/DCX Polyclonal Antibody
😃Good
Catalog No. TMAB-00564
Alias XLIS, SCLH, LISX, doublecortin, DC, DBCN
Anti-Doublecortin/DCX Polyclonal Antibody is a Rabbit antibody targeting Doublecortin/DCX. Anti-Doublecortin/DCX Polyclonal Antibody can be used in WB,ICC/IF.
Anti-Doublecortin/DCX Polyclonal Antibody
Anti-Doublecortin/DCX Polyclonal Antibody
😃Good
Catalog No. TMAB-00564Alias XLIS, SCLH, LISX, doublecortin, DC, DBCN
Anti-Doublecortin/DCX Polyclonal Antibody is a Rabbit antibody targeting Doublecortin/DCX. Anti-Doublecortin/DCX Polyclonal Antibody can be used in WB,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Doublecortin/DCX Polyclonal Antibody is a Rabbit antibody targeting Doublecortin/DCX. Anti-Doublecortin/DCX Polyclonal Antibody can be used in WB,ICC/IF. |
| Alias | XLIS, SCLH, LISX, doublecortin, DC, DBCN |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Rat) Lysate at 40 μg Primary: Anti-DCX/Doublecortin (TMAB-00564) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa 2. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (DCX/Doublecortin) polyclonal Antibody, Unconjugated (TMAB-00564) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Cell projection. Note=Localizes at neurite tips. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Neuronal Marker Doublecortin (DCX) is a microtubule-associated protein expressed almost exclusively in immature neurons. Neuronal precursors begin to express DCX shortly after exiting the cell cycle, and continue to express DCX for 2-3 weeks as the cells mature into neurons. Downregulation of DCX begins after 2 weeks, and occurs at the same time that these cells begin to express, a marker for mature neurons. Due to the nearly exclusive expression of DCX in developing neurons, this protein has been used increasingly as a marker for neurogenesis. Indeed, the levels of DCX expression increase in response to exercise, which occurs in parallel with increased BrdU labelling, currently a "gold standard" in measuring neurogenesis. |
| Immunogen | KLH conjugated synthetic peptide: human DCX |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Microtubules,Growth Cone,Soma marker,Neuronal |
| Molecular Weight | Theoretical: 49 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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