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Anti-CTLA4 Polyclonal Antibody
😃Good
Catalog No. TMAB-00491
Alias IDDM12, ICOS, GSE, GRD4, CTLA4, CELIAC3, CD152, ALPS5
Anti-CTLA4 Polyclonal Antibody is a Rabbit antibody targeting CTLA4. Anti-CTLA4 Polyclonal Antibody can be used in WB,FCM.
Anti-CTLA4 Polyclonal Antibody
Anti-CTLA4 Polyclonal Antibody
😃Good
Catalog No. TMAB-00491Alias IDDM12, ICOS, GSE, GRD4, CTLA4, CELIAC3, CD152, ALPS5
Anti-CTLA4 Polyclonal Antibody is a Rabbit antibody targeting CTLA4. Anti-CTLA4 Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CTLA4 Polyclonal Antibody is a Rabbit antibody targeting CTLA4. Anti-CTLA4 Polyclonal Antibody can be used in WB,FCM. |
| Alias | IDDM12, ICOS, GSE, GRD4, CTLA4, CELIAC3, CD152, ALPS5 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Blank control: Raji (blue). Primary Antibody: Rabbit Anti-CTLA4 antibody (TMAB-00491), Dilution: 5 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 2. Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min) Isotype Control Antibody: Rabbit Igg (orange); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA; Primary Antibody Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA (green). 3. Blank control (blue): Mouse Spleen Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Phospho-c-Fos (Thr325)/FITC Conjugated antibody (TMAB-00491/FITC), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC (orange),used under the same conditions. 4. Blank control (blue): Molt-4 Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CTLA4/FITC Conjugated antibody (TMAB-00491/FITC), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC orange),used under the same conditions. 5. Sample: A549 (Human) Cell Lysate at 30 μg Primary: Anti-CTLA4 (TMAB-00491) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 35 kDa 6. Sample: Liver (Mouse) Cell Lysate at 30 μg Primary: Anti-CTLA4 (TMAB-00491) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 35 kDa 7. Sample: Liver (Rat) Cell Lysate at 30 μg Primary: Anti-CTLA4 (TMAB-00491) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 35 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human CTLA-4/CD152 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Regulatory T Cells,CD,Cytotoxic Cells,Autoimmune,SARS Coronavirus,T Lymphocytic Lineage |
| Molecular Weight | Theoretical: 21 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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