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Anti-CDK1 Antibody (2M625)
😃Good
Catalog No. TMAC-00782
Alias P34CDC2, cyclin-dependent kinase 1, CDC28A, CDC2
Anti-CDK1 Antibody (2M625) is a Rabbit antibody targeting CDK1. Anti-CDK1 Antibody (2M625) can be used in WB,ICC,IHC,FCM.
Anti-CDK1 Antibody (2M625)
Anti-CDK1 Antibody (2M625)
😃Good
Catalog No. TMAC-00782Alias P34CDC2, cyclin-dependent kinase 1, CDC28A, CDC2
Anti-CDK1 Antibody (2M625) is a Rabbit antibody targeting CDK1. Anti-CDK1 Antibody (2M625) can be used in WB,ICC,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-CDK1 Antibody (2M625) is a Rabbit antibody targeting CDK1. Anti-CDK1 Antibody (2M625) can be used in WB,ICC,IHC,FCM. |
| Synonyms | P34CDC2, cyclin-dependent kinase 1, CDC28A, CDC2 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of CDK1 on Jurkat cells lysates using anti-CDK1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CDK1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDK1 antibody. Counter stained with hematoxylin. 4. ICC staining CDK1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining CDK1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CDK1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Jurkat cells with CDK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin-dependent kinase 2 (Cdk2) p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2 p34-related cyclin dependent kinases have been identified. These include Cdk3-Cdk8, PCTAIRE-1-3 and KKIALRE. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 34 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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