Anti-CD11c Polyclonal Antibody is a Rabbit antibody targeting CD11c. Anti-CD11c Polyclonal Antibody can be used in FCM.

IgG

Human,Mouse (predicted:Rat,Dog)

1. Blank control (blue): U937 cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD11c antibody (TMAB-00331), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange) used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
2. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CD11c antibody (TMAB-00331)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CD11c antibody (TMAB-00331)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Integrin Alpha X

Human

Monocytic Lineage,Dendritic Cell Lineage,T Lymphocytic Lineage,Alpha,Adhesion