Anti-Beta-Catenin Polyclonal Antibody is a Rabbit antibody targeting Beta-Catenin. Anti-Beta-Catenin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Primary Antibody: Anti-Beta catenin Polyclonal Antibody, Unconjugated (TMAB-00230) 1:200, overnight at 4°C
Secondary Antibody:
IHC: Goat Anti-Rabbit IgG, Biotin conjugated + Streptavidin , HRP conjugated , and DAb staining.
IF: Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C, Excitation wavelength: 565nm; Emission wavelength:578nm
2. Tissue/cell: rat skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Primary Antibody: Anti-Beta catenin Polyclonal Antibody, Unconjugated (TMAB-00230) 1:200, overnight at 4°C
Secondary Antibody:
IHC: Goat Anti-Rabbit IgG, Biotin conjugated + Streptavidin , HRP conjugated , and DAb staining.
IF: Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C, Excitation wavelength: 565nm; Emission wavelength:578nm
3. Tissue/cell: human esophageal cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Beta catenin Polyclonal Antibody, Unconjugated (TMAB-00230) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Beta catenin) Polyclonal Antibody, Unconjugated (TMAB-00230) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
5. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-Beta catenin (TMAB-00230) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 9.5/86 kDa
Observed band size: 86 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Beta catenin

Human

Cytoplasmic,Catenins,Cadherins,beta catenin,Neurogenesis,ChIP antibodies,Leukocyte recruitment,Cadherins