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Anti-5HT2C Receptor Polyclonal Antibody
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Catalog No. TMAB-00010
Anti-5HT2C Receptor Polyclonal Antibody is a Rabbit antibody targeting 5HT2C Receptor. Anti-5HT2C Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-5HT2C Receptor Polyclonal Antibody
Anti-5HT2C Receptor Polyclonal Antibody
😃Good
Catalog No. TMAB-00010
Anti-5HT2C Receptor Polyclonal Antibody is a Rabbit antibody targeting 5HT2C Receptor. Anti-5HT2C Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-5HT2C Receptor Polyclonal Antibody is a Rabbit antibody targeting 5HT2C Receptor. Anti-5HT2C Receptor Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with 5HTR2C Polyclonal Antibody, Unconjugated (TMAB-00010) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Human SH-SY5Y cell lysates Lane 2: Human A431 cell lysates Primary: Anti-5HT2C Receptor (TMAB-00010) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 59 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in humans have reported abnormalities in patterns of 5-HT2C editing in depressed suicide victims. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human 5HTR2C |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Obesity,Metabolism,Serotonin Receptors,GPCR |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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