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Anti-14-3-3 gamma Antibody (5D580)
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Catalog No. TMAC-00004
Alias YWHAG, Protein kinase C inhibitor protein 1 (KCIP-1), KCIP1, 1433G
Anti-14-3-3 gamma Antibody (5D580) is a Rabbit antibody targeting 14-3-3 gamma. Anti-14-3-3 gamma Antibody (5D580) can be used in FCM,WB.
Anti-14-3-3 gamma Antibody (5D580)
Anti-14-3-3 gamma Antibody (5D580)
😃Good
Catalog No. TMAC-00004Alias YWHAG, Protein kinase C inhibitor protein 1 (KCIP-1), KCIP1, 1433G
Anti-14-3-3 gamma Antibody (5D580) is a Rabbit antibody targeting 14-3-3 gamma. Anti-14-3-3 gamma Antibody (5D580) can be used in FCM,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-14-3-3 gamma Antibody (5D580) is a Rabbit antibody targeting 14-3-3 gamma. Anti-14-3-3 gamma Antibody (5D580) can be used in FCM,WB. |
| Synonyms | YWHAG, Protein kinase C inhibitor protein 1 (KCIP-1), KCIP1, 1433G |
| Ig Type | IgG |
| Clone | 5D580 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of 14-3-3 gamma on different lysates using anti-14-3-3 gamma antibody at 1/1,000 dilution. Positive control: Lane 1: 293T, Lane 2: A431 Lane 3: Hela. 2. Flow cytometric analysis of K562 cells with 14-3-3 gamma antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.   |
| Application | |
| Recommended Dose | WB: 1:1000-2000; FCM: 1:10-50 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 b, g, e, z, h, q and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins; by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity, and facilitation of protein modification. Loss of expression contributes to a vast array of pathogenic cellular activities. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 28 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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