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Alpha-Tubulin Loading Control Antibody (8C509) is a Mouse antibody targeting Alpha-Tubulin. Alpha-Tubulin Loading Control Antibody (8C509) can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
| Description | Alpha-Tubulin Loading Control Antibody (8C509) is a Mouse antibody targeting Alpha-Tubulin. Alpha-Tubulin Loading Control Antibody (8C509) can be used in WB. |
| Synonyms | α-tubulin, Tubulin-alpha, Tubulin H2-alpha, Tubulin alpha 4A chain, Tubulin alpha 4a, Tubulin alpha 1(testis specific), Tubulin alpha 1 chain, Tubulin alpha 1, Tuba4a, TUBA1, TUBA 4A, Testis-specific alpha-tubulin, TBA4A, H2 alpha, FLJ30169, Detyrosinated alpha Tubulin, alpha-tubulin 1 |
| Ig Type | IgG |
| Clone | 8C509 |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Rabbit,Zebrafish,GuineaPig) |
| Verified Activity | Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse NIH/3T3 cell lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Human HeLa cell lysates Lane 5: Human Jurkat cell lysates Lane 6: Human A431 cell lysates Lane 7: Human U251 cell lysates Lane 8: Human 293T cell lysates Lane 9: Human HepG2 cell lysates Primary: Anti-alpha Tubulin (TMAB-00108) at 1/100000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa ![]() |
| Application | |
| Recommended Dose | WB=1:5000-100000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm, cytoskeleton. |
| Tissue Specificity | Widely expressed. Overexpressed in Platelet, Brain, and Frontal cortex |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Tubulin is a major cytoskeleton that has five distinct forms, designated alpha, beta, gamma, delta and epsilon tubulin. The alpha and beta tubulins form a heterodimer that polymerize into the cylindrical microtubule fibers. Both alpha and beta tubulin bind GTP. Only beta tubulin hydrolyzes GTP to GDP. This hydrolysis is a process that is linked to tubulin polymerization and microtubule formation. The alpha tubulin isomer can be modified by addition of a C-terminal tyrosine residue. This modification may influence polymerization rates. The gamma tubulin isomer is localized to centrosomes which compose the heart of the microtubule organizing center from which microtubule fibers emanate. |
| Immunogen | Recombinant Protein: human alpha Tubulin Protein |
| Antigen Species | Human |
| Gene Name | TUBA1A |
| Gene ID | |
| Protein Name | Tubulin alpha-1A chain |
| Uniprot ID | |
| Biology Area | Tubulin |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain |
| Molecular Weight | Theoretical: 50 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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