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Alpha-Tubulin Loading Control Antibody (8C509)
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Catalog No. TMAB-00108
Alias α-tubulin, Tubulin-alpha, Tubulin H2-alpha, Tubulin alpha 4A chain, Tubulin alpha 4a, Tubulin alpha 1(testis specific), Tubulin alpha 1 chain, Tubulin alpha 1, Tuba4a, TUBA1, TUBA 4A, Testis-specific alpha-tubulin, TBA4A, H2 alpha, FLJ30169, Detyrosinated alpha Tubulin, alpha-tubulin 1
Alpha-Tubulin Loading Control Antibody (8C509) is a Mouse antibody targeting Alpha-Tubulin Loading Control. Alpha-Tubulin Loading Control Antibody (8C509)
Alpha-Tubulin Loading Control Antibody (8C509)
Alpha-Tubulin Loading Control Antibody (8C509)
😃Good
Catalog No. TMAB-00108Alias α-tubulin, Tubulin-alpha, Tubulin H2-alpha, Tubulin alpha 4A chain, Tubulin alpha 4a, Tubulin alpha 1(testis specific), Tubulin alpha 1 chain, Tubulin alpha 1, Tuba4a, TUBA1, TUBA 4A, Testis-specific alpha-tubulin, TBA4A, H2 alpha, FLJ30169, Detyrosinated alpha Tubulin, alpha-tubulin 1
Alpha-Tubulin Loading Control Antibody (8C509) is a Mouse antibody targeting Alpha-Tubulin Loading Control. Alpha-Tubulin Loading Control Antibody (8C509)
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Alpha-Tubulin Loading Control Antibody (8C509) is a Mouse antibody targeting Alpha-Tubulin Loading Control. Alpha-Tubulin Loading Control Antibody (8C509) |
| Synonyms | α-tubulin, Tubulin-alpha, Tubulin H2-alpha, Tubulin alpha 4A chain, Tubulin alpha 4a, Tubulin alpha 1(testis specific), Tubulin alpha 1 chain, Tubulin alpha 1, Tuba4a, TUBA1, TUBA 4A, Testis-specific alpha-tubulin, TBA4A, H2 alpha, FLJ30169, Detyrosinated alpha Tubulin, alpha-tubulin 1 |
| Ig Type | IgG |
| Clone | 8C509 |
| Reactivity | Human,Mouse,Rat,Zebrafish (predicted:Chicken,Dog,Pig,Rabbit,GuineaPig) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha Tubulin) Monoclonal Antibody, Unconjugated (ascites of TMAB-00108) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse NIH/3T3 cell lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Human HeLa cell lysates Lane 5: Human Jurkat cell lysates Lane 6: Human A431 cell lysates Lane 7: Human U251 cell lysates Lane 8: Human 293T cell lysates Lane 9: Human HepG2 cell lysates Primary: Anti-alpha Tubulin (TMAB-00108) at 1/100000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa  |
| Application | |
| Recommended Dose | WB: 1:50000-200000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm, cytoskeleton. |
| Tissue Specificity | Widely expressed. Overexpressed in Platelet, Brain, and Frontal cortex |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] |
| Immunogen | Recombinant Protein: human alpha Tubulin Protein |
| Antigen Species | Human |
| Gene Name | TUBA4A |
| Gene ID | |
| Protein Name | Tubulin alpha-4A chain |
| Uniprot ID | |
| Biology Area | Tubulin |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain |
| Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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