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Alpha-Tubulin Loading Control Antibody (8C509)

😃Good
Catalog No. TMAB-00108
Alias α-tubulin, Tubulin-alpha, Tubulin H2-alpha, Tubulin alpha 4A chain, Tubulin alpha 4a, Tubulin alpha 1(testis specific), Tubulin alpha 1 chain, Tubulin alpha 1, Tuba4a, TUBA1, TUBA 4A, Testis-specific alpha-tubulin, TBA4A, H2 alpha, FLJ30169, Detyrosinated alpha Tubulin, alpha-tubulin 1

Alpha-Tubulin Loading Control Antibody (8C509) is a Mouse antibody targeting Alpha-Tubulin Loading Control. Alpha-Tubulin Loading Control Antibody (8C509) can be used in WB,IHC-P,IHC-Fr,IF.

Alpha-Tubulin Loading Control Antibody (8C509)

Alpha-Tubulin Loading Control Antibody (8C509)

😃Good
Catalog No. TMAB-00108Alias α-tubulin, Tubulin-alpha, Tubulin H2-alpha, Tubulin alpha 4A chain, Tubulin alpha 4a, Tubulin alpha 1(testis specific), Tubulin alpha 1 chain, Tubulin alpha 1, Tuba4a, TUBA1, TUBA 4A, Testis-specific alpha-tubulin, TBA4A, H2 alpha, FLJ30169, Detyrosinated alpha Tubulin, alpha-tubulin 1
Alpha-Tubulin Loading Control Antibody (8C509) is a Mouse antibody targeting Alpha-Tubulin Loading Control. Alpha-Tubulin Loading Control Antibody (8C509) can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Alpha-Tubulin Loading Control Antibody (8C509) is a Mouse antibody targeting Alpha-Tubulin Loading Control. Alpha-Tubulin Loading Control Antibody (8C509) can be used in WB,IHC-P,IHC-Fr,IF.
Aliasα-tubulin, Tubulin-alpha, Tubulin H2-alpha, Tubulin alpha 4A chain, Tubulin alpha 4a, Tubulin alpha 1(testis specific), Tubulin alpha 1 chain, Tubulin alpha 1, Tuba4a, TUBA1, TUBA 4A, Testis-specific alpha-tubulin, TBA4A, H2 alpha, FLJ30169, Detyrosinated alpha Tubulin, alpha-tubulin 1
Ig Type
IgG
Clone
8C509
Reactivity
Human,Mouse,Rat,Zebrafish (predicted:Chicken,Dog,Pig,Rabbit,GuineaPig)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (alpha Tubulin) Monoclonal Antibody, Unconjugated (ascites of TMAB-00108) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse NIH/3T3 cell lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human Jurkat cell lysates
Lane 6: Human A431 cell lysates
Lane 7: Human U251 cell lysates
Lane 8: Human 293T cell lysates
Lane 9: Human HepG2 cell lysates
Primary: Anti-alpha Tubulin (TMAB-00108) at 1/100000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:50000-200000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasm, cytoskeleton.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundMicrotubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]
Antigen Details
Immunogen
Recombinant Protein: human alpha Tubulin Protein
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Tubulin
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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