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Abz-FRLKGGAPIKGV-EDDNP TFA
🥰Excellent
Catalog No. T208580
Abz-FRLKGGAPIKGV-EDDNP TFA is a fluorescent substrate used to assess the enzymatic activity of proteases, such as the papain-like protease 2 (PLP2) found in severe SARS-CoV. It shows potential for research into 2019-nCoV (COVID-19) infection.
Abz-FRLKGGAPIKGV-EDDNP TFA
Abz-FRLKGGAPIKGV-EDDNP TFA
🥰Excellent
Catalog No. T208580
Abz-FRLKGGAPIKGV-EDDNP TFA is a fluorescent substrate used to assess the enzymatic activity of proteases, such as the papain-like protease 2 (PLP2) found in severe SARS-CoV. It shows potential for research into 2019-nCoV (COVID-19) infection.
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Product Introduction
Bioactivity
Chemical Properties
| Description | Abz-FRLKGGAPIKGV-EDDNP TFA is a fluorescent substrate used to assess the enzymatic activity of proteases, such as the papain-like protease 2 (PLP2) found in severe SARS-CoV. It shows potential for research into 2019-nCoV (COVID-19) infection. |
| In vitro | Abz-FRLKGGAPIKGV-EDDNP TFA exhibits minimal fluorescent emission due to fluorescence resonance energy transfer (FRET) between Abz and EDDNP. At pH 7.4, the apparent k_cat and K_m values for this fluorogenic substrate are 20 min^-1 and 77 μM, respectively. Substrate cleavage results in increased fluorescence emission, detected at excitation and emission wavelengths of 320 and 420 nm, respectively, whether or not the chemical compounds are present. |
Storage & Solubility Information
| Storage | keep away from direct sunlight | store at -20°C | Shipping with blue ice/Shipping at ambient temperature. |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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