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8pyDTZ

Name: 8pyDTZ
Brand: TargetMol
SKU: T19167
Rating: 4.5 (1 reviews)

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Catalog No. T19167Cas No. 2351898-91-2

8pyDTZ is a pyridyl diphenylterazine (DTZ) analog and an ATP-independent pyridyl substrate of LumiLuc luciferase, exhibiting spectrally shifted emission and improved water solubility, suitable for in vivo luminescence imaging.

8pyDTZ

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Catalog No. T19167Cas No. 2351898-91-2

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$1,330	6-8 weeks	6-8 weeks
50 mg	$1,740	6-8 weeks	6-8 weeks
100 mg	$2,500	6-8 weeks	6-8 weeks

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Product Introduction

Bioactivity

Description	8pyDTZ is a pyridyl diphenylterazine (DTZ) analog and an ATP-independent pyridyl substrate of LumiLuc luciferase, exhibiting spectrally shifted emission and improved water solubility, suitable for in vivo luminescence imaging.
In vitro	8pyDTZ, with a solubility of 1711 μM, significantly increases water solubility by 13-fold over DTZ (131 μM). This compound not only shows a capability for red-shifted chemiluminescence and bioluminescence emission compared to DTZ but also produces the most red-shifted emission, allowing for improved tissue penetration. In evaluations using human embryonic kidney 293T cells expressing luciferase (HEK 293T cells), the LumiLuc-8pyDTZ combination yields approximately 3-5 times more bioluminescence than the teLuc-8pyDTZ pair. Additionally, when comparing far-red emission intensities with a 600-700 nm bandpass filter, LumiLuc-8pyDTZ consistently achieves 1.6-3.9 times higher photon flux than teLuc-DTZ across substrate concentrations ranging from 6.25 to 100 μM, without affecting ATP levels in LumiLuc-expressing cells treated with 8pyDTZ.
In vivo	The LumiLuc-8pyDTZ pair was evaluated alongside various standard reporters within a tumor xenograft mouse model, exhibiting high sensitivity and eliminating the need for organic cosolvents for in vivo administration. Additionally, LumiScarlet-8pyDTZ demonstrated equivalence to Akaluc-AkaLumine, the most luminous ATP-dependent luciferase-luciferin pair, in detecting cells within deep mouse tissues.

Chemical Properties

Molecular Weight	378.43
Formula	C₂₄H₁₈N₄O
Cas No.	2351898-91-2
Smiles	O=c1c(Cc2ccccc2)nc2c([nH]c(cn12)-c1ccccc1)-c1ccncc1
Relative Density.	1.26 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

EtOH+HCl: 2 mg/mL (5.28 mM), when pH is adjusted to 2. Sonication is recommended. (DMSO inactivates the activity of 8pyDTZ.)

Solution Preparation Table

EtOH+HCl

	1mg	5mg	10mg	50mg
1 mM	2.6425 mL	13.2125 mL	26.4250 mL	132.1248 mL
5 mM	0.5285 mL	2.6425 mL	5.2850 mL	26.4250 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

8pyDTZ