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4-Nitrophenyl β-D-Galactopyranoside

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Catalog No. T65845Cas No. 3150-24-1

4-Nitrophenyl β-D-Galactopyranoside is a β-D-galactopyranoside derivative that is a substrate for β-galactosidase and can be used to measure β-galactosidase activity, which can be used in the study of lysosomal storage diseases, obesity, and mucopolysaccharides storage diseases.

4-Nitrophenyl β-D-Galactopyranoside

4-Nitrophenyl β-D-Galactopyranoside

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Purity: 99.91%
Catalog No. T65845Cas No. 3150-24-1
4-Nitrophenyl β-D-Galactopyranoside is a β-D-galactopyranoside derivative that is a substrate for β-galactosidase and can be used to measure β-galactosidase activity, which can be used in the study of lysosomal storage diseases, obesity, and mucopolysaccharides storage diseases.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
1 g$29-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Batch Information

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Purity:99.91%
Appearance:Solid
Color:White
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Product Introduction

Bioactivity
Description
4-Nitrophenyl β-D-Galactopyranoside is a β-D-galactopyranoside derivative that is a substrate for β-galactosidase and can be used to measure β-galactosidase activity, which can be used in the study of lysosomal storage diseases, obesity, and mucopolysaccharides storage diseases.
Chemical Properties
Molecular Weight301.25
FormulaC12H15NO8
Cas No.3150-24-1
SmilesO=[N+]([O-])c1ccc(O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)cc1
Storage & Solubility Information
Storagestore under nitrogen,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
DMSO: 80 mg/mL (265.56 mM), Sonication is recommended.
H2O: 2 mg/mL (6.64 mM), Sonication is recommended.
In Vivo Formulation
10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (16.6 mM), Sonication is recommended.
Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
Solution Preparation Table
H2O/DMSO
1mg5mg10mg50mg
1 mM3.3195 mL16.5975 mL33.1950 mL165.9751 mL
5 mM0.6639 mL3.3195 mL6.6390 mL33.1950 mL
DMSO
1mg5mg10mg50mg
10 mM0.3320 mL1.6598 mL3.3195 mL16.5975 mL
20 mM0.1660 mL0.8299 mL1.6598 mL8.2988 mL
50 mM0.0664 mL0.3320 mL0.6639 mL3.3195 mL
100 mM0.0332 mL0.1660 mL0.3320 mL1.6598 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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