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4-Azido-L-phenylalanine

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Catalog No. T14038Cas No. 33173-53-4
Alias p-Azidophenylalanine, p-Azido-L-phenylalanine

4-Azido-L-phenylalanine (p-Azido-L-phenylalanine) is an amino acid derivative that acts as a photoaffinity marker and can be used to detect localized protein environments.

4-Azido-L-phenylalanine

4-Azido-L-phenylalanine

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Purity: 99.24%
Catalog No. T14038Alias p-Azidophenylalanine, p-Azido-L-phenylalanineCas No. 33173-53-4
4-Azido-L-phenylalanine (p-Azido-L-phenylalanine) is an amino acid derivative that acts as a photoaffinity marker and can be used to detect localized protein environments.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
10 mg$30In StockIn Stock
25 mg$43In StockIn Stock
50 mg$59In StockIn Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Purity:99.24%
Appearance:Solid
Color:Yellow
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Product Introduction

Bioactivity
Description
4-Azido-L-phenylalanine (p-Azido-L-phenylalanine) is an amino acid derivative that acts as a photoaffinity marker and can be used to detect localized protein environments.
In vitro
Within the cell-free protein synthesis (CFPS) method, the CFPS solutions accumulate modified soluble super-folder green fluorescent protein (sfGFP) at concentrations ranging from 0.9 to 1.7 mg/mL, including either 4-Azido-L-phenylalanine or p-propargyloxy-l-phenylalanine (pPaF).[1]
Synonymsp-Azidophenylalanine, p-Azido-L-phenylalanine
Chemical Properties
Molecular Weight206.2
FormulaC9H10N4O2
Cas No.33173-53-4
SmilesN[C@@H](CC1=CC=C(C=C1)N=[N+]=[N-])C(O)=O
Relative Density.no data available
Storage & Solubility Information
Storagestore at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
H2O: 22.5 mg/mL (109.12 mM), when pH is adjusted to 11 with NaOH. Sonication is recommended.
DMSO: 5 mg/mL (24.25 mM), pH is adjusted to 3 with HCl.
Solution Preparation Table
DMSO/H2O
1mg5mg10mg50mg
1 mM4.8497 mL24.2483 mL48.4966 mL242.4830 mL
5 mM0.9699 mL4.8497 mL9.6993 mL48.4966 mL
10 mM0.4850 mL2.4248 mL4.8497 mL24.2483 mL
20 mM0.2425 mL1.2124 mL2.4248 mL12.1242 mL
H2O
1mg5mg10mg50mg
50 mM0.0970 mL0.4850 mL0.9699 mL4.8497 mL
100 mM0.0485 mL0.2425 mL0.4850 mL2.4248 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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