1,8-Diamino-4,5-dihydroxyanthraquinone serves as a multifunctional dye with extensive applications in both traditional and emerging fields. As critical components in biological experiments, dyes enable researchers to examine and analyze cellular structures, track biomolecules, assess cellular functions, differentiate cell types, detect biomolecules, investigate tissue pathology, and monitor microorganisms. Their utility spans from fundamental scientific research to a broad spectrum of clinical diagnostics. Additionally, these compounds are pivotal in industries like textile dyeing and are advancing into areas including functional textile processing, food pigments, and dye-sensitized solar cells.