ED 50 < 0.2 ng/ml, measured in ability to inhibit the mouse IL-4-dependent proliferation of HT-2 cells.

TGF-beta 1 Protein, Bovine, Recombinant is expressed in CHO Cells. The accession number is P18341.

Bovine

CHO Cells

transforming growth factor beta-1,TGF-beta-1,TGF-beta 1 protein,TGF-b1,TGFB1,TGFB,TGF beta,latency-associated peptide,DPD1,CEDLAP,CED

Ala279-Ser390

≥ 95% as determined by SDS-PAGE

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH 2 O or 50 mM Citrate up to 100 μg/ml.

Upon receiving, this product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product should be stable for up to 1 week at 4 °C or up to 3 months at -20 °C. For long-term storage after reconstitution, it is recommended that a carrier protein (e.g., 0.1% BSA) be added. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 °C.

TGF-β1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-β1 superfamily that share a characteristic cystine knot structure. TGF-β1, -2 and -3 are highly pleiotropic cytokines that act as cellular switches to regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-β isoform has some non-redundant function; for TGF-β1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation and died of overwhelming inflammation. TGF-β1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-β RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-β RI (also called activin receptor‑like kinase (ALK)-5), or alternatively, ALK-1. This complex phosphorylates and activates Smad proteins that regulate transcription.