SARS-CoV-2 Spike RBD Protein (K417N & E484K & N501Y, His), Biotinylated

Name: SARS-CoV-2 Spike RBD Protein (K417N & E484K & N501Y, His), Biotinylated
Brand: TargetMol
SKU: TMPY-06118
Rating: 4.5 (1 reviews)

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Catalog No. TMPY-06118 Copy Product Info

SARS-CoV-2 Spike RBD Protein (K417N & E484K & N501Y, His), Biotinylated is expressed in HEK293 mammalian cells. The predicted molecular weight is 26.7 kDa and the accession number is A0A6G7K2L4.

Pack Size	Price	USA Stock	Global Stock
5 μg	$250	7-10 days	7-10 days
10 μg	$419	7-10 days	7-10 days
20 μg	$812	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	SARS-CoV-2 Spike RBD Protein (K417N & E484K & N501Y, His), Biotinylated is expressed in HEK293 mammalian cells. The predicted molecular weight is 26.7 kDa and the accession number is A0A6G7K2L4.
Species	SARS-CoV-2
Expression System	HEK293 Cells
Tag	C-His
Accession Number	A0A6G7K2L4
Construction	A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD (YP_009724390.1, with mutations K417N, E484K, N501Y) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro. The mutations were identified in the SARS-CoV-2 variant (known as 20C/501Y.V2 or B.1.351 lineage) which emerged in South Africa. Predicted N terminal: Ser
Protein Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background

Chemical Properties

Molecular Weight

26.7 kDa (predicted); 36 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Storage

Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: SARS-CoV-2 Spike RBD Protein (K417N & E484K & N501Y, His), Biotinylated chemical structure | SARS-CoV-2 Spike RBD Protein (K417N & E484K & N501Y, His), Biotinylated molecular weight