PIN1 Protein, Human, Recombinant

Name: PIN1 Protein, Human, Recombinant
Brand: TargetMol
SKU: TMPY-02114
Rating: 4.5 (1 reviews)

(Synonyms: UBL5, peptidylprolyl cis/trans isomerase, NIMA-interacting 1, DOD) Copy Product Info

Synonyms: UBL5, peptidylprolyl cis/trans isomerase, NIMA-interacting 1, DOD

Catalog No. TMPY-02114 Copy Product Info

PIN1 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18.2 kDa and the accession number is Q13526-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$85	7-10 days	7-10 days
10 µg	$146	7-10 days	7-10 days
20 µg	$242	7-10 days	7-10 days
50 µg	$477	7-10 days	7-10 days
100 µg	$929	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	PIN1 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18.2 kDa and the accession number is Q13526-1.
Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	Q13526-1
Construction	A DNA sequence encoding the mature form of human PIN1 (Q13526-1) (Met 1-Glu 163) was expressed and purified. Predicted N terminal: Met
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 10% glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	UBL5, peptidylprolyl cis/trans isomerase, NIMA-interacting 1, DOD
Research Background	Peptidyl-prolyl cis-trans isomerase Pin1, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Rotamase Pin1 and PIN1, peptidyl-prolyl cis/trans isomerase (PPIase), is a nucleus protein. PIN1 is a peptidyl-prolyl isomerase that can alter the conformation of phosphoproteins and so affect protein function and/or stability. PIN1 regulates a number of proteins important for cell-cycle progression and is presumed to operate as a molecular timer of this important process. PIN1 is an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. PIN1 displays a preference for an acidic residue N-terminal to the isomerized proline bond. Alterations in the level of PIN1 can influence hyperproliferative diseases such as cancer. PIN1 has been implicated in multiple aspects of cell cycle regulation. It has been suggested that PIN1 function is required for both normal mitotic progression and reentry into the cell cycle from quiescence. PIN1 is also a target of several oncogenic pathways and is overexpressed in human breast cancer. Its overexpression can lead to upregulation of cyclin-D1 and transformation of breast epithelial cells in collaboration with the oncogenic pathways. PIN1 plays a pivotal role in breast development and may be a promising new anticancer target. Pin1 activity regulates the outcome of proline-directed kinase (e.g. MAPK, CDK or GSK3) signalling and consequently regulates cell proliferation (in part through control of cyclin D1 levels and stability) and cell survival. Recent data also implicate Pin1 as playing an important role in immune responses, at least in part by increasing the stability of cytokine mRNAs by influencing the protein complexes to which they bind.

Chemical Properties

Molecular Weight

18.2 kDa (predicted); 18 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.