Insulin Protein, Bovine, Recombinant

Name: Insulin Protein, Bovine, Recombinant
Brand: TargetMol
SKU: TMPS-00095
Rating: 4.5 (1 reviews)

(Synonyms: Insulin) Copy Product Info

Synonyms: Insulin

Catalog No. TMPS-00095 Copy Product Info

Insulin Protein, Bovine, Recombinant is expressed in P. pastoris (Yeast). The accession number is P01317.

Pack Size	Price	USA Stock	Global Stock
25 mg	$377	7-10 days	7-10 days
50 mg	$633	7-10 days	7-10 days
100 mg	$1,070	7-10 days	7-10 days
200 mg	$1,760	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	ED 50 < 1.00 μg/ml as determined by a dose-response proliferation assay using human MCF7 epithelial cells. Based on the ED 50 , the calculated specific activity is approximately > 1× 10 3 IU/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.
Description	Insulin Protein, Bovine, Recombinant is expressed in P. pastoris (Yeast). The accession number is P01317.
Species	Bovine
Expression System	P. pastoris (Yeast)
Tag	Tag Free
Accession Number	P01317
Construction	Phe25-Ala54 & Gly85-Asn105
Protein Purity	≥ 95% as determined by SDS-PAGE
Endotoxin	< 0.1 EU/μg of protein as determined by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered solution in 10 mM Citric acid, pH 2.0-3.0.
Reconstitution	Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH 2 O up to 1 mg/ml.
Synonyms	Insulin
Research Background	Insulin is a peptide hormone produced exclusively by beta cells of the pancreatic islets. The mature form of insulin is a heterodimer of a B chain and an A chain linked by two disulfide bonds. The amino acid sequence of insulin is strongly conserved and varies only slightly between species. Bovine insulin differs from human in only three amino acid residues, and porcine insulin in one. Insulin is considered to be the main anabolic hormone of the body and regulates the metabolism of carbohydrates, fats and protein by promoting the absorption of glucose from the blood into liver, fat and skeletal muscle cells. Decreased or absent insulin activity results in diabetes. Recombinant insulin has been widely used for treatment of diabetes.

Chemical Properties

Molecular Weight

5.72 kDa (Predicted); 6 kDa (Reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 °C. Upon reconstitution, the product is stable for up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 °C.